A Noisy Analog-to-Digital Converter Connects Cytosolic Calcium Bursts to Transcription Factor Nuclear Localization Pulses in Yeast.
Crz1
calcineurin pathway
mathematical modelling
stochastic pulsing transcription factor
time delay model
Journal
G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598
Informations de publication
Date de publication:
07 02 2019
07 02 2019
Historique:
pubmed:
24
12
2018
medline:
5
6
2019
entrez:
22
12
2018
Statut:
epublish
Résumé
Several examples of transcription factors that show stochastic, unsynchronized pulses of nuclear localization have been described. Here we show that under constant calcium stress, nuclear localization pulses of the transcription factor Crz1 follow stochastic variations in cytosolic calcium concentration. We find that the size of the stochastic calcium bursts is positively correlated with the number of subsequent Crz1 pulses. Based on our observations, we propose a simple stochastic model of how the signaling pathway converts a constant external calcium concentration into a digital number of Crz1 pulses in the nucleus, due to the time delay from nuclear transport and the stochastic decoherence of individual Crz1 molecule dynamics. We find support for several additional predictions of the model and suggest that stochastic input to nuclear transport may produce noisy digital responses to analog signals in other signaling systems.
Identifiants
pubmed: 30573469
pii: g3.118.200841
doi: 10.1534/g3.118.200841
pmc: PMC6385971
doi:
Substances chimiques
CRZ1 protein, S cerevisiae
0
DNA-Binding Proteins
0
Saccharomyces cerevisiae Proteins
0
Transcription Factors
0
Banques de données
figshare
['10.25387/g3.7485575']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
561-570Informations de copyright
Copyright © 2019 Hsu et al.
Références
J Biol Chem. 1999 Feb 26;274(9):5939-47
pubmed: 10026219
EMBO J. 2001 Mar 15;20(6):1320-30
pubmed: 11250898
J Cell Biol. 2002 Jan 7;156(1):29-34
pubmed: 11781332
J Biol Chem. 2002 Aug 23;277(34):31079-88
pubmed: 12058033
J Mol Biol. 2003 Mar 14;327(1):31-45
pubmed: 12614606
Biochem Biophys Res Commun. 2003 Nov 28;311(4):1143-50
pubmed: 14623300
Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14593-8
pubmed: 16199522
Cell Calcium. 2006 Apr;39(4):337-48
pubmed: 16445978
Mol Syst Biol. 2006;2:2006.0033
pubmed: 16773083
Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17418-23
pubmed: 17954914
Cell Calcium. 2009 Feb;45(2):123-32
pubmed: 18783827
Nature. 2008 Sep 25;455(7212):485-90
pubmed: 18818649
Microbiology. 2008 Dec;154(Pt 12):3775-81
pubmed: 19047745
Mol Cell. 2009 Mar 13;33(5):616-26
pubmed: 19285944
Science. 2009 Apr 10;324(5924):242-6
pubmed: 19359585
Biophys J. 2009 Sep 2;97(5):1244-53
pubmed: 19720012
Phys Rev E Stat Nonlin Soft Matter Phys. 2009 Aug;80(2 Pt 1):021909
pubmed: 19792153
Nat Methods. 2009 Dec;6(12):875-81
pubmed: 19898485
Methods Enzymol. 2011;498:349-61
pubmed: 21601685
Annu Rev Cell Biol. 1990;6:715-60
pubmed: 2177344
Science. 2011 Oct 21;334(6054):366-9
pubmed: 21979936
Cell Calcium. 2012 Jan;51(1):72-81
pubmed: 22153127
Science. 2012 Jun 15;336(6087):1440-4
pubmed: 22700930
J Biol Chem. 2012 Nov 23;287(48):40246-55
pubmed: 23071118
Mol Cell. 2013 Jan 24;49(2):249-61
pubmed: 23219535
PLoS One. 2013;8(1):e53404
pubmed: 23335962
PLoS Comput Biol. 2013;9(6):e1003112
pubmed: 23825938
Science. 2013 Dec 6;342(6163):1193-200
pubmed: 24311681
Nat Methods. 2014 Mar;11(3):319-24
pubmed: 24487582
Mol Cell. 2014 Aug 7;55(3):422-435
pubmed: 24930733
Methods Mol Biol. 2014;1205:79-90
pubmed: 25213240
Curr Biol. 2014 Sep 22;24(18):2189-2194
pubmed: 25220054
PLoS One. 2014 Sep 19;9(9):e107766
pubmed: 25238410
Fungal Biol Rev. 2014 Oct;28(2-3):56-69
pubmed: 25383089
Elife. 2015 May 18;4:null
pubmed: 25985085
Cell Calcium. 2015 Aug;58(2):226-35
pubmed: 26055636
Nature. 2015 Nov 5;527(7576):54-8
pubmed: 26466562
J Physiol. 2016 Jun 15;594(12):3317-38
pubmed: 26824875
Curr Genet. 1989 Dec;16(5-6):339-46
pubmed: 2692852
Curr Biol. 2016 Apr 4;26(7):R269-71
pubmed: 27046808
Elife. 2016 Sep 30;5:
pubmed: 27690227
Mol Biol Cell. 2017 Feb 15;28(4):501-510
pubmed: 28031257
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1456-1461
pubmed: 28154146
Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):E1450-E1459
pubmed: 28167781
PLoS Comput Biol. 2017 May 11;13(5):e1005479
pubmed: 28493880
J Biol Chem. 2017 Jul 28;292(30):12366-12372
pubmed: 28637875
Cell. 2018 Feb 8;172(4):869-880.e19
pubmed: 29398116
Biochem Biophys Res Commun. 1986 Oct 15;140(1):320-8
pubmed: 3022718
J Biol Chem. 1986 Dec 5;261(34):16115-20
pubmed: 3023342
J Biol Chem. 1984 Jul 25;259(14):8847-56
pubmed: 6086614
Math Biosci. 1998 Sep;152(2):143-63
pubmed: 9780612