IFT20 is required for the maintenance of cartilaginous matrix in condylar cartilage.
Chondrocyte
Condyle
Golgi
Intraflagellar transport
Mice
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
29 01 2019
29 01 2019
Historique:
received:
10
12
2018
accepted:
14
12
2018
pubmed:
28
12
2018
medline:
9
10
2019
entrez:
28
12
2018
Statut:
ppublish
Résumé
Condylar cartilage is a joint cartilage essential for smooth jaw movement. The importance of ciliary proteins in condylar cartilage development has been reported. However, little is known about how ciliary proteins control the homeostasis of condylar cartilage. Here we show that intraflagellar transport 20 (IFT20), a ciliary protein, is required for the maintenance of cartilaginous matrix in condylar cartilage. Utilizing NG2-CreER mice expressed in condylar cartilage, we deleted Ift20 by tamoxifen treatment at juvenile-to-adult stages. In wild-type condylar cartilage, IFT20 was robustly produced in the cis-Golgi, but deletion of Ift20 by tamoxifen induction of NG2-CreER (Ift20:NG2-CreER) resulted in reduced cell proliferation and decreased Golgi size in condylar cartilage. Importantly, while the primary cilia were present in cartilage cells in the condylar layers of wild-type mice, no primary cilia were present in the Ift20:NG2-CreER condylar layers. Consistent with this finding, ciliary-mediated Hedgehog signaling was severely attenuated in Ift20 mutant chondrocytes, and thus the production levels of type X collagen were significantly reduced in Ift20:NG2-CreER mice. These results suggest that IFT20 is required for Golgi size and Hedgehog signaling to maintain cartilaginous matrix in condylar cartilage. Our study highlights the unique function of IFT20 in the homeostasis of condylar cartilage.
Identifiants
pubmed: 30587338
pii: S0006-291X(18)32756-6
doi: 10.1016/j.bbrc.2018.12.107
pmc: PMC6443088
mid: NIHMS1517378
pii:
doi:
Substances chimiques
Carrier Proteins
0
Hedgehog Proteins
0
Ift20 protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
222-226Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR054465
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR070222
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE025897
Pays : United States
Organisme : NIAMS NIH HHS
ID : R21 AR060978
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.
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