Chloroquine Inhibits Self-Renewal of Blast Progenitors Synergistically With Phytochemicals or Nonsteroidal Anti-inflammatory Drugs in Hematological Malignant Cell Lines.
Anti-Inflammatory Agents, Non-Steroidal
/ pharmacology
Antineoplastic Combined Chemotherapy Protocols
/ pharmacology
Ascorbic Acid
/ pharmacology
Blast Crisis
/ drug therapy
Cell Line, Tumor
Cell Self Renewal
/ drug effects
Chloroquine
/ pharmacology
Hematologic Neoplasms
/ drug therapy
Humans
Indomethacin
/ pharmacology
Neoplastic Stem Cells
Nitrobenzenes
/ pharmacology
Phytochemicals
/ pharmacology
Resveratrol
/ pharmacology
Stem Cells
/ drug effects
Sulfonamides
/ pharmacology
Tumor Stem Cell Assay
Chloroquine
NSAIDs
blast progenitor self-renewal
hematological malignancies
phytochemicals
synergistic inhibition
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
28
10
2018
revised:
30
11
2018
accepted:
04
12
2018
entrez:
29
12
2018
pubmed:
29
12
2018
medline:
8
1
2019
Statut:
ppublish
Résumé
This study examined whether and how chloroquine inhibits blast progenitor self-renewal (SR) synergistically with phytochemicals or nonsteroidal anti-inflammatory drugs in seven hematological malignant cell lines. Vitamin C, resveratrol, cyclo-oxygenase inhibitor NS-398 and indomethacin heptyl ester (Ind) were added to cell culture with or without 3 μM chloroquine. Chloroquine synergistically inhibited blast colony formation in methylcellulose with vitamin C, resveratrol, NS-398 and Ind in one, two, none and one cell lines, respectively, in a total of four out of 28 conditions. Chloroquine synergistically inhibited blast progenitor SR in suspension with vitamin C, resveratrol, NS-398 and Ind in four, six, one and five cell lines, respectively, in a total of 16 out of 28 conditions. In contrast, chloroquine abolished SR inhibition by another agent in four out of 28 conditions. Chloroquine exerted a marked synergistic inhibition of blast progenitor SR, but not blast colony formation.
Sections du résumé
BACKGROUND
BACKGROUND
This study examined whether and how chloroquine inhibits blast progenitor self-renewal (SR) synergistically with phytochemicals or nonsteroidal anti-inflammatory drugs in seven hematological malignant cell lines.
MATERIALS AND METHODS
METHODS
Vitamin C, resveratrol, cyclo-oxygenase inhibitor NS-398 and indomethacin heptyl ester (Ind) were added to cell culture with or without 3 μM chloroquine.
RESULTS
RESULTS
Chloroquine synergistically inhibited blast colony formation in methylcellulose with vitamin C, resveratrol, NS-398 and Ind in one, two, none and one cell lines, respectively, in a total of four out of 28 conditions. Chloroquine synergistically inhibited blast progenitor SR in suspension with vitamin C, resveratrol, NS-398 and Ind in four, six, one and five cell lines, respectively, in a total of 16 out of 28 conditions. In contrast, chloroquine abolished SR inhibition by another agent in four out of 28 conditions.
CONCLUSION
CONCLUSIONS
Chloroquine exerted a marked synergistic inhibition of blast progenitor SR, but not blast colony formation.
Identifiants
pubmed: 30591444
pii: 39/1/87
doi: 10.21873/anticanres.13083
doi:
Substances chimiques
Anti-Inflammatory Agents, Non-Steroidal
0
Nitrobenzenes
0
Phytochemicals
0
Sulfonamides
0
N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
123653-11-2
Chloroquine
886U3H6UFF
Ascorbic Acid
PQ6CK8PD0R
Resveratrol
Q369O8926L
Indomethacin
XXE1CET956
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
87-98Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.