Strong electrostatic repulsive interaction used for fast and effective alkaloid enrichment from plants.

Alkaloids / analysis Chromatography, Ion Exchange / methods Chromatography, Reverse-Phase / methods Plant Extracts / chemistry Scopolia / chemistry Static Electricity
Alkaloid enrichment Class separation Electrostatic repulsion Mixed-mode stationary phase Reversed-phase/strong anion-exchange Scopolia tangutica

Journal

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554

Informations de publication

Date de publication:
15 Jan 2019
Historique:
received: 20 09 2018
revised: 19 11 2018
accepted: 18 12 2018
pubmed: 31 12 2018
medline: 12 2 2019
entrez: 31 12 2018
Statut: ppublish

Résumé

Since the content of alkaloids is usually low in plants and they are easily co-eluted with other constituents, enrichment of alkaloids is essential in the discovery of bioactive lead compounds from natural products. In this paper, an easy SPE enrichment method was developed in a buffer-free solvent system based on electrostatic repulsion mechanism. The feasibility of the new method was verified by successful enrichment of alkaloids from Scopolia tangutica (S. tangutica) with an optimized eluting condition. Then this developed method was applied to other representative plants in different families, including Przewalskia tangutica and Peganum harmala L, Lycoris radiata and Menispermum dauricum DC, which enlarged the scope of applicability. Additionally, the new SPE procedure avoided possible structural change destruction caused by pH change. Simple solvent system, including formic acid (FA) and methanol, would benefit subsequent mass analysis, quantity determination and bioactivity screening, and so on.

Identifiants

DOI: 10.1016/j.jchromb.2018.12.024 PMID: 30594825
pubmed: 30594825
pii: S1570-0232(18)31447-8
doi: 10.1016/j.jchromb.2018.12.024
pii:
doi:

Substances chimiques

Alkaloids 0
Plant Extracts 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

148-155

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Nana Du (N)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China; University of Chinese Academy of Sciences, Beijing, China.

Wei Si (W)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Yanfang Liu (Y)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. Electronic address: liuyanfang@dicp.ac.cn.

Zhimou Guo (Z)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Weijia Zhou (W)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China; University of Chinese Academy of Sciences, Beijing, China.

Han Zhou (H)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Xinmiao Liang (X)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. Electronic address: liangxm@dicp.ac.cn.

Chaoran Wang (C)

Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

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Classifications MeSH

questionsmedicales.fr › Composés hétérocycliques › Alcaloïdes › Strong electrostatic repulsive interaction...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'échange d'ions › Strong electrostatic repulsive interaction...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase inverse › Strong electrostatic repulsive interaction...
questionsmedicales.fr › Préparations pharmaceutiques › Extraits de plantes › Strong electrostatic repulsive interaction...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Solanales › Solanaceae › Scopolia › Strong electrostatic repulsive interaction...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électricité › Électricité statique › Strong electrostatic repulsive interaction...