Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis.
RNA
Sox2
Wnt
cell cycle
dermal condensate
dermis
hair follicle
morphogenesis
single-cell
β-catenin
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
07 01 2019
07 01 2019
Historique:
received:
31
05
2018
revised:
18
09
2018
accepted:
16
11
2018
pubmed:
1
1
2019
medline:
2
8
2019
entrez:
1
1
2019
Statut:
ppublish
Résumé
Delineating molecular and cellular events that precede appendage morphogenesis has been challenging due to the inability to distinguish quantitative molecular differences between cells that lack histological distinction. The hair follicle (HF) dermal condensate (DC) is a cluster of cells critical for HF development and regeneration. Events that presage emergence of this distinctive population are poorly understood. Using unbiased single-cell RNA sequencing and in vivo methods, we infer a sequence of transcriptional states through which DC cells pass that begins prior to HF morphogenesis. Our data indicate that Wnt/β-catenin signaling is required to progress into an intermediate stage that precedes quiescence and differentiation. Further, we provide evidence that quiescent DC cells are recent progeny of selectively proliferating cells present prior to morphogenesis and that are later identified in the peri-DC zone during DC expansion. Together, these findings provide an inferred path of molecular states that lead to DC cell differentiation.
Identifiants
pubmed: 30595533
pii: S1534-5807(18)30988-2
doi: 10.1016/j.devcel.2018.11.032
pmc: PMC6361530
mid: NIHMS1514149
pii:
doi:
Substances chimiques
beta Catenin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
17-31.e6Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM131642
Pays : United States
Organisme : NIH HHS
ID : S10 OD018521
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG008383
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR076420
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007205
Pays : United States
Organisme : NIAMS NIH HHS
ID : K08 AR066790
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR068392
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR071491
Pays : United States
Organisme : NIAMS NIH HHS
ID : R03 AR072807
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.
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