Effects of fusion-activation interval and embryo aggregation on in vitro and in vivo development of bovine cloned embryos.
Embryo aggregation, fusion-activation interval
Nuclear transfer
Journal
Research in veterinary science
ISSN: 1532-2661
Titre abrégé: Res Vet Sci
Pays: England
ID NLM: 0401300
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
30
01
2018
revised:
16
11
2018
accepted:
13
12
2018
pubmed:
1
1
2019
medline:
10
5
2019
entrez:
1
1
2019
Statut:
ppublish
Résumé
Nuclear reprogramming in somatic cell cloning is one of the key factors for proper development, with variations in the protocol appearing to improve cloning efficiency. This study aimed to determine the effects of two fusion-activation intervals and the aggregation of bovine cloned embryos on subsequent in vitro and in vivo development. Zygotes produced by handmade cloning were exposed to two fusion-activation intervals (2 h or 4 h), and then cultured in microwells either individually (1 × 100%) or after aggregation of two structures (2 × 100%). Zona-intact oocytes and zona-free oocytes and hemi-oocytes were used as parthenote controls under the same fusion-activation intervals. Day-7 cloned blastocysts were transferred to synchronous recipients. Cleavage (Day 2), blastocyst (Day 7) and pregnancy (Day 30) rates were compared by the χ
Identifiants
pubmed: 30597478
pii: S0034-5288(18)30088-2
doi: 10.1016/j.rvsc.2018.12.001
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
91-98Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.