Titre : Techniques de culture d'embryons

Techniques de culture d'embryons : Questions médicales fréquentes

Questions fréquentes et termes MeSH associés

Diagnostic 2

#1

Comment évaluer la qualité des embryons cultivés ?

La qualité des embryons est évaluée par leur morphologie et leur développement cellulaire.
Embryons Culture d'embryons
#2

Quels tests sont utilisés pour diagnostiquer des anomalies embryonnaires ?

Des tests génétiques comme le CGH-Array et le séquençage sont utilisés pour détecter des anomalies.
Anomalies chromosomiques Diagnostic génétique

Symptômes 2

#1

Quels symptômes peuvent indiquer un problème de culture embryonnaire ?

Des symptômes comme l'absence de développement ou des anomalies morphologiques peuvent indiquer des problèmes.
Développement embryonnaire Anomalies embryonnaires
#2

Les embryons mal cultivés peuvent-ils causer des symptômes ?

Non, les embryons mal cultivés ne causent pas de symptômes chez la mère, mais peuvent affecter la grossesse.
Culture d'embryons Grossesse

Prévention 2

#1

Comment prévenir les anomalies lors de la culture d'embryons ?

Utiliser des milieux de culture optimisés et surveiller les conditions environnementales aide à prévenir les anomalies.
Prévention des anomalies Culture d'embryons
#2

Quels facteurs environnementaux influencent la culture embryonnaire ?

La température, l'humidité et la composition du milieu de culture influencent le développement embryonnaire.
Environnement de culture Développement embryonnaire

Traitements 2

#1

Quelles techniques améliorent la culture d'embryons ?

Des techniques comme la culture en milieu contrôlé et l'utilisation de co-cultures améliorent les résultats.
Techniques de culture Procréation assistée
#2

Comment la cryoconservation aide-t-elle la culture d'embryons ?

La cryoconservation permet de préserver les embryons pour une utilisation future, augmentant les chances de succès.
Cryoconservation Embryons

Complications 2

#1

Quelles complications peuvent survenir lors de la culture d'embryons ?

Des complications comme la défaillance de développement ou la contamination peuvent survenir.
Complications de la culture Contamination
#2

Les embryons mal cultivés peuvent-ils affecter la grossesse ?

Oui, des embryons mal cultivés peuvent entraîner des échecs d'implantation ou des fausses couches.
Échec d'implantation Fausses couches

Facteurs de risque 2

#1

Quels facteurs augmentent le risque d'échec de culture embryonnaire ?

L'âge maternel avancé, des antécédents de fausses couches et des problèmes de fertilité augmentent le risque.
Âge maternel Fertilité
#2

Comment le mode de vie influence-t-il la culture d'embryons ?

Le tabagisme, l'alcool et une mauvaise alimentation peuvent nuire à la qualité des embryons cultivés.
Mode de vie Qualité embryonnaire
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améliorent la culture d'embryons ?", "position": 7, "acceptedAnswer": { "@type": "Answer", "text": "Des techniques comme la culture en milieu contrôlé et l'utilisation de co-cultures améliorent les résultats." } }, { "@type": "Question", "name": "Comment la cryoconservation aide-t-elle la culture d'embryons ?", "position": 8, "acceptedAnswer": { "@type": "Answer", "text": "La cryoconservation permet de préserver les embryons pour une utilisation future, augmentant les chances de succès." } }, { "@type": "Question", "name": "Quelles complications peuvent survenir lors de la culture d'embryons ?", "position": 9, "acceptedAnswer": { "@type": "Answer", "text": "Des complications comme la défaillance de développement ou la contamination peuvent survenir." } }, { "@type": "Question", "name": "Les embryons mal cultivés peuvent-ils affecter la grossesse ?", "position": 10, "acceptedAnswer": { "@type": "Answer", "text": "Oui, des embryons mal cultivés peuvent entraîner des échecs d'implantation ou des fausses couches." } }, { "@type": "Question", "name": "Quels facteurs augmentent le risque d'échec de culture embryonnaire ?", "position": 11, "acceptedAnswer": { "@type": "Answer", "text": "L'âge maternel avancé, des antécédents de fausses couches et des problèmes de fertilité augmentent le risque." } }, { "@type": "Question", "name": "Comment le mode de vie influence-t-il la culture d'embryons ?", "position": 12, "acceptedAnswer": { "@type": "Answer", "text": "Le tabagisme, l'alcool et une mauvaise alimentation peuvent nuire à la qualité des embryons cultivés." } } ] } ] }
Dr Olivier Menir

Contenu validé par Dr Olivier Menir

Expert en Médecine, Optimisation des Parcours de Soins et Révision Médicale


Validation scientifique effectuée le 26/04/2026

Contenu vérifié selon les dernières recommandations médicales

Auteurs principaux

Peter J Hansen

2 publications dans cette catégorie

Affiliations :
  • Department of Animal Sciences, University of Florida, Gainesville, FL, USA. pjhansen@ufl.edu.
Publications dans "Techniques de culture d'embryons" :

Hongmei Wang

2 publications dans cette catégorie

Affiliations :
  • State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Publications dans "Techniques de culture d'embryons" :

Yu Yin

2 publications dans cette catégorie

Affiliations :
  • State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
  • Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan, China.
  • Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, China.
Publications dans "Techniques de culture d'embryons" :

Lifeng Xiang

2 publications dans cette catégorie

Affiliations :
  • Department of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Publications dans "Techniques de culture d'embryons" :

Gaohui Shi

2 publications dans cette catégorie

Affiliations :
  • State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
  • Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan, China.
Publications dans "Techniques de culture d'embryons" :

Tianqing Li

2 publications dans cette catégorie

Affiliations :
  • State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China. litq@lpbr.cn.
  • Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan, China. litq@lpbr.cn.
  • Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, China. litq@lpbr.cn.
Publications dans "Techniques de culture d'embryons" :

Nicholas D E Greene

2 publications dans cette catégorie

Affiliations :
  • Newlife Birth Defects Research Centre, UCL GOS Institute of Child Health, University College London, London, United Kingdom.
Publications dans "Techniques de culture d'embryons" :

Andrew J Copp

2 publications dans cette catégorie

Affiliations :
  • Newlife Birth Defects Research Centre, UCL GOS Institute of Child Health, University College London, London, United Kingdom.
Publications dans "Techniques de culture d'embryons" :

Barbara J Stone

1 publication dans cette catégorie

Affiliations :
  • ParaTechs Corporation, Lexington, Kentucky;, Email: barbarastone@paratechs.com.
Publications dans "Techniques de culture d'embryons" :

Kendra H Steele

1 publication dans cette catégorie

Affiliations :
  • ParaTechs Corporation, Lexington, Kentucky.
Publications dans "Techniques de culture d'embryons" :

Hongsheng Men

1 publication dans cette catégorie

Affiliations :
  • Rat Resource and Research Center, University of Missouri, Columbia, Missouri; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri.
Publications dans "Techniques de culture d'embryons" :

Sarah J Srodulski

1 publication dans cette catégorie

Affiliations :
  • ParaTechs Corporation, Lexington, Kentucky.
Publications dans "Techniques de culture d'embryons" :

Elizabeth C Bryda

1 publication dans cette catégorie

Affiliations :
  • Rat Resource and Research Center, University of Missouri, Columbia, Missouri; Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri.
Publications dans "Techniques de culture d'embryons" :

Angelika Fath-Goodin

1 publication dans cette catégorie

Affiliations :
  • ParaTechs Corporation, Lexington, Kentucky.
Publications dans "Techniques de culture d'embryons" :

Sotirios H Saravelos

1 publication dans cette catégorie

Affiliations :
  • IVF Unit, Hammersmith Hospital, Imperial College, London, UK. Electronic address: s.saravelos@imperial.ac.uk.
Publications dans "Techniques de culture d'embryons" :

Tin-Chiu Li

1 publication dans cette catégorie

Affiliations :
  • Assisted Reproductive Technology Unit, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong. Electronic address: tinchiu.li@cuhk.edu.hk.
Publications dans "Techniques de culture d'embryons" :

Emily W Y Tung

1 publication dans cette catégorie

Affiliations :
  • Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
Publications dans "Techniques de culture d'embryons" :

Louise M Winn

1 publication dans cette catégorie

Affiliations :
  • Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. winnl@queensu.ca.
  • School of Environmental Studies, Queen's University, Kingston, ON, Canada. winnl@queensu.ca.
Publications dans "Techniques de culture d'embryons" :

Michael T Kane

1 publication dans cette catégorie

Affiliations :
  • Department of Physiology, National University of Ireland, Galway, Ireland. michael.kane@nuigalway.ie.
Publications dans "Techniques de culture d'embryons" :

Islam M Saadeldin

1 publication dans cette catégorie

Affiliations :
  • Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia. isaadeldin@ksu.edu.sa.
  • Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt. isaadeldin@ksu.edu.sa.
Publications dans "Techniques de culture d'embryons" :

Sources (10000 au total)

Successful pregnancy with intracytoplasmic sperm injection after bacterial contamination of embryo culture in in vitro fertilization: a case report.

Bacterial infection of embryo culture medium is rare but may be detrimental. The main source of embryo culture contamination is semen. Assisted reproduction centers currently lack consensus regarding ... We present a case report of two consecutive in vitro fertilization-intracytoplasmic sperm injection cycles with photo and video documentation of the bacterial growth. A 36-year-old Hungarian woman and... Intracytoplasmic sperm injection may be offered to couples who fail conventional in vitro fertilization treatment owing to bacteriospermia, as it seems to prevent infection of the embryo culture. Even...

Can time-lapse culture combined with artificial intelligence improve ongoing pregnancy rates in fresh transfer cycles of single cleavage stage embryos?

With the rapid advancement of time-lapse culture and artificial intelligence (AI) technologies for embryo screening, pregnancy rates in assisted reproductive technology (ART) have significantly improv... This retrospective study analyzed 105 fresh embryo transfer cycles at the Centre for Reproductive Medicine from August 2023 to March 2024, following IVF/ICSI treatment at the cleavage stage. All embry... Baseline characteristics such as patient age, AMH levels, AFC, and basal sex hormones showed no significant differences among the three groups (p > 0.05). The iDAscores were significantly higher in Gr... Combining time-lapse culture with AI scoring may enhance ongoing pregnancy rates in single cleavage-stage fresh transfer cycles....

Randomized prospective study comparing conventional In Vitro Fertilization technique to Intravaginal Culture with the INVOCELL™ device for 3 and 5 days.

The objective was to analyse and compare the formation and quality of the embryos developed using conventional in vitro fertilization (IVF) and IVC techniques with an INVOCell™ device.... Two groups were formed, with eight couples in each, one in culture for three days (D3) and another in culture for five days (D5), using intravaginal culture technique with an INVOcell device and a con... Embryo formation in Group D5 showed 46.7% (IVC) and 40% (IVF) of recovered blastocysts. In the group D5, the conventional IVF, better embryo development dynamics was observed, with 66% of expanded bla... The results showed that the IVC technique, using the INVOCell™ device, provided a healthy and balanced environment for the development and obtaining of quality embryos with three and five days of cult...

Humidified atmosphere in a time-lapse embryo culture system does not improve ongoing pregnancy rate: a retrospective propensity score model study derived from 496 first ICSI cycles.

To investigate whether high relative humidity conditions (HC), when using a time-lapse system (TLS) with sequential culture media, are beneficial to embryo culture, improving ongoing pregnancy rates.... We included patients undergoing their first ICSI cycle treatment from April 2021 to May 2022. Patients assigned to dry conditions (DC) or HC were 278 and 218, respectively. We used a GERI TLS, three c... After adjusting for several confounding variables and applying the propensity score (PS), no significant differences were observed in the rates of normal (2PN) and abnormal (1PN and 3PN) fertilization... These results suggest that HC conditions do not improve the rate of ongoing pregnancy and several embryological outcomes, under the conditions used in this study based on a time-lapse system and seque...

Application of amphiregulin in IVM culture of immature human oocytes and pre-insemination culture for COCs in IVF cycles.

Amphiregulin (AR) is a growth factor that resembles the epidermal growth factor (EGF) and serves various functions in different cells. However, no systematic studies or reports on the role of AR in hu... First, we examined the concentration of AR in the follicular fluid (FF) of patients who underwent routine IVF and explored the correlation between AR levels and oocyte maturation and subsequent embryo... A total of 609 FF samples were examined, and a positive correlation between AR levels and blastocyst formation was observed. In our IVM study, the development potential and IVM rate of immature oocyte... AR levels in the FF positively correlated with blastocyst formation, and AR involvement in pre-insemination cultures of COCs can effectively improve laboratory outcomes in IVF. Furthermore, AR can dir...

Delineating the heterogeneity of embryo preimplantation development using automated and accurate morphokinetic annotation.

Our objective was to design an automated deep learning model that extracts the morphokinetic events of embryos that were recorded by time-lapse incubators. Using automated annotation, we set out to ch... To perform a retrospective study, we used a dataset of video files of 67,707 embryos from four IVF clinics. A convolutional neural network (CNN) model was trained to assess the developmental states th... We perform automated assessment of single-frame embryo states with 97% accuracy and demonstrate whole-embryo morphokinetic annotation with R-square 0.994. High quality embryos that had been valid cand... By demonstrating fully automated, accurate, and standardized morphokinetic annotation of time-lapse embryo recordings from IVF clinics, we provide practical means to overcome current limitations that ...

Altered morphokinetics and differential reproductive outcomes associated with cell exclusion events in human embryos.

Can embryos harbouring cell exclusion and their reproductive outcomes be classified based on morphokinetic profiles?... A total of 469 time-lapse videos of embryos transferred between 2013 and 2019 from a single clinic were analysed. Videos were assessed and grouped according to the presence or absence of one or more e... Transfer of CE+ embryos resulted in lower proportions of fetal heartbeat (FHB) and live birth compared with CE- embryos (both, P < 0.001). CE+ embryos were associated with delays in t2 (P = 0.030), t6... Embryos harbouring cell exclusion indicated altered morphokinetic profiles. Their overall lower reproductive success was associated with two morphokinetic parameters. Morphokinetic profiles could be u...

A novel machine-learning framework based on early embryo morphokinetics identifies a feature signature associated with blastocyst development.

Artificial Intelligence entails the application of computer algorithms to the huge and heterogeneous amount of morphodynamic data produced by Time-Lapse Technology. In this context, Machine Learning (... We retrospectively analysed the morphokinetics of 575 embryos obtained from 80 women who underwent IVF at our Unit. Embryo morphokinetics was registered using the Geri plus® time-lapse system. Overall... The novel EmbryoMLSelection framework was developed following four-steps: Feature Selection, Rules Extraction, Rules Selection and Rules Evaluation. Six rules composed by a combination of 8 variables ... We provided herein a new feature-signature able to identify with an high performance embryos with the best developmental competence to reach the expanded blastocyst stage on day 5. Clear and clinicall...