Evaluation of 4 Presurgical Skin Preparation Methods in Mice.
Journal
Journal of the American Association for Laboratory Animal Science : JAALAS
ISSN: 2769-6677
Titre abrégé: J Am Assoc Lab Anim Sci
Pays: United States
ID NLM: 101269489
Informations de publication
Date de publication:
01 01 2019
01 01 2019
Historique:
pubmed:
6
1
2019
medline:
7
2
2020
entrez:
6
1
2019
Statut:
ppublish
Résumé
Mice routinely undergo surgical procedures for use in research; however, studies of skin preparation methods to achieve antisepsis are rare. The present study evaluated 4 skin preparation treatments: depilatory agent followed by povidone-iodine and alcohol scrub; depilatory agent followed by povidone-iodine and saline scrub; electric clippers followed by povidone-iodine and alcohol scrub; and electric clippers followed by povidone-iodine and saline scrub. Swabs for bacterial culture were obtained immediately after hair removal and after scrubbing to measure the reduction in bacterial load. Full-thickness incisions were assigned ASEPSIS wound scores and examined histologically on days 0, 1, and 7 after surgery. Neither bacterial load growth nor ASEPSIS wound scores differed among any of the treatments. Histopathology revealed statistically significant but biologically irrelevant differences. Overall all treatment methods achieved acceptable bacterial load reduction and surgical site healing.
Identifiants
pubmed: 30609948
doi: 10.30802/AALAS-JAALAS-18-000047
pmc: PMC6351048
doi:
Substances chimiques
Anti-Infective Agents, Local
0
Chlorhexidine
R4KO0DY52L
Ethanol
3K9958V90M
Povidone-Iodine
85H0HZU99M
Types de publication
Clinical Trial, Veterinary
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
71-77Subventions
Organisme : NIH HHS
ID : P51 OD011132
Pays : United States
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