Purification and biochemical characterization of a novel acido-halotolerant and thermostable endochitinase from Melghiribacillus thermohalophilus strain Nari2A

Bacillaceae / enzymology Chitin / metabolism Chitinases / isolation & purification Enzyme Stability / drug effects Hydrogen-Ion Concentration Hydrolysis Kinetics Metals / pharmacology Molecular Weight Sodium Chloride / pharmacology Solvents / pharmacology Substrate Specificity Temperature
Chitin Chitinase Hydrolytic pattern Melghiribacillus thermohalophilus Thin-layer chromatography

Journal

Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535

Informations de publication

Date de publication:
01 Feb 2019
Historique:
received: 29 11 2018
revised: 27 12 2018
accepted: 28 12 2018
pubmed: 8 1 2019
medline: 6 5 2019
entrez: 8 1 2019
Statut: ppublish

Résumé

An extracellular acido-thermostable endochitinase (called ChiA-Mt45) from thermohalophilic Melghiribacillus thermohalophilus strain Nari2A

Identifiants

DOI: 10.1016/j.carres.2018.12.017 PMID: 30616169
pubmed: 30616169
pii: S0008-6215(18)30695-5
doi: 10.1016/j.carres.2018.12.017
pii:
doi:

Substances chimiques

Metals 0
Solvents 0
Chitin 1398-61-4
Sodium Chloride 451W47IQ8X
Chitinases EC 3.2.1.14

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

46-56

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Sara Mohamed (S)

Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Khelifa Bouacem (K)

Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria; Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax, 3018, Tunisia. Electronic address: kbouacem@usthb.dz.

Sondes Mechri (S)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax, 3018, Tunisia.

Nariman Ammara Addou (NA)

Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Hassiba Laribi-Habchi (H)

Laboratory of Functional Analysis of Chemical Processes (LFACP), Process Engineering Department, Faculty of Technology, University of Blida 1, Road of Soumaâ, PO Box 270, 09000, Blida, Algeria.

Marie-Laure Fardeau (ML)

Aix Marseille University, IRD, University of Toulon, CNRS, Mediterranean Institute of Oceanography (MIO), UM 110, ESIL Box 925, 163 Avenue of Luminy, 13288, Marseille Cedex 9, France.

Bassem Jaouadi (B)

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax, 3018, Tunisia.

Amel Bouanane-Darenfed (A)

Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.

Hocine Hacène (H)

Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria. Electronic address: h_hacene@yahoo.fr.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Purification and biochemical characterization...
questionsmedicales.fr › Glucides › Polyosides › Chitine › Purification and biochemical characterization...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Chitinase › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Purification and biochemical characterization...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Masse moléculaire › Purification and biochemical characterization...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du sodium › Chlorure de sodium › Purification and biochemical characterization...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Solvants › Purification and biochemical characterization...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Purification and biochemical characterization...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Purification and biochemical characterization...