Extraction and characterization of stratified extracellular polymeric substances in Geobacter biofilms.
Bioelectrochemical system
Extracellular electron transport
Extracellular polymeric substances
Extraction protocol
Geobacter biofilm
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
13
11
2018
revised:
25
12
2018
accepted:
26
12
2018
pubmed:
8
1
2019
medline:
20
8
2019
entrez:
8
1
2019
Statut:
ppublish
Résumé
Extracellular polymeric substances (EPS) play crucial roles in promoting biofilm formation and contribute to electrochemical activities of biofilms in bioelectrochemical systems (BES). In this study, three stratified EPS fractions were extracted from Geobacter biofilms using EDTA-, ultrasound- and heating-based protocols and characterized with chemical, spectral and electrochemical analyses. Results suggested that, for Geobacter biofilms, ultrasound-based extraction protocol was more effective in EPS yield (62.1-66.5 mg C/g dry cell) than EDTA method, and had less cell lysis than heating method. The extraction methods greatly affected the proteins composition in the extracted EPS, indicated by the varied ratios of tryptophan/tyrosine protein-like substances. Electrochemical measurements demonstrated a good correlation between protein concentration and extracellular electron transfer function for both tightly-bound EPS and total EPS. This is the first study to extract and characterize stratified EPS fractions from Geobacter biofilms, and helpful for better understanding the function of EPS in BESs predominated by Geobacter.
Identifiants
pubmed: 30616210
pii: S0960-8524(18)31771-1
doi: 10.1016/j.biortech.2018.12.100
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
119-126Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.