Production and characterization of a monoclonal antibody for Pefloxacin and mechanism study of antibody recognition.
Animals
Anti-Bacterial Agents
/ analysis
Antibodies, Monoclonal
/ biosynthesis
Antibody Affinity
Antibody Specificity
Binding Sites
Binding, Competitive
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
/ methods
Escherichia coli
/ genetics
Gene Expression
Hybridomas
/ chemistry
Immunization
Immunoconjugates
/ administration & dosage
Immunoglobulin Variable Region
/ biosynthesis
Limit of Detection
Mice
Mice, Inbred BALB C
Molecular Docking Simulation
Mutation
Pefloxacin
/ analysis
Protein Binding
Protein Structure, Secondary
Recombinant Proteins
/ biosynthesis
Serum Albumin, Bovine
/ chemistry
Structural Homology, Protein
ELISA
Pefloxacin
molecular recognition
monoclonal antibody
Journal
Bioscience, biotechnology, and biochemistry
ISSN: 1347-6947
Titre abrégé: Biosci Biotechnol Biochem
Pays: England
ID NLM: 9205717
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
pubmed:
9
1
2019
medline:
2
4
2019
entrez:
9
1
2019
Statut:
ppublish
Résumé
In this report, an artificial antigen (PFLX-BSA: Pefloxacin connected bovine serum albumin) was successfully prepared. The monoclonal antibody against pefloxacin was produced and characterized using a direct competitive ELISA. The linear range of detection was 0.115-6.564 µg/L. The limit of detection defined as IC
Identifiants
pubmed: 30618329
doi: 10.1080/09168451.2018.1562876
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antibodies, Monoclonal
0
Immunoconjugates
0
Immunoglobulin Variable Region
0
Recombinant Proteins
0
Serum Albumin, Bovine
27432CM55Q
Pefloxacin
2H52Z9F2Q5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM