Mechanistic aspects of maltotriose-conjugate translocation to the Gram-negative bacteria cytoplasm.


Journal

Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869

Informations de publication

Date de publication:
02 2019
Historique:
received: 13 11 2018
revised: 19 12 2018
accepted: 19 12 2018
entrez: 9 1 2019
pubmed: 9 1 2019
medline: 9 1 2019
Statut: epublish

Résumé

Small molecule accumulation in Gram-negative bacteria is a key challenge to discover novel antibiotics, because of their two membranes and efflux pumps expelling toxic molecules. An approach to overcome this challenge is to hijack uptake pathways so that bacterial transporters shuttle the antibiotic to the cytoplasm. Here, we have characterized maltodextrin-fluorophore conjugates that can pass through both the outer and inner membranes mediated by components of the

Identifiants

pubmed: 30620010
doi: 10.26508/lsa.201800242
pii: LSA-2018-00242
pmc: PMC6311466
doi:

Substances chimiques

Bacterial Outer Membrane Proteins 0
Escherichia coli Proteins 0
MalE protein, E coli 0
Maltose-Binding Proteins 0
Membrane Transport Proteins 0
Oligosaccharides 0
Periplasmic Binding Proteins 0
Polysaccharides 0
Porins 0
Receptors, Virus 0
Trisaccharides 0
maltoporins 0
maltohexaose 34620-77-4
Perylene 5QD5427UN7
maltotriose 639K0T34IK
Maltose 69-79-4
maltodextrin 7CVR7L4A2D

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e201800242

Déclaration de conflit d'intérêts

The author (RA Stavenger) declares competing financial interests: RA Stavenger is an employee and shareholder of GlaxoSmithKline.

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Auteurs

Estelle Dumont (E)

Aix Marseille Univ, Institut National de la Santé et de la Recherche Médicale, Service de Santé des Armées, Institut de Recherche Biomédicale des Armées, Membranes et Cibles Thérapeutiques, Marseille, France.

Julia Vergalli (J)

Aix Marseille Univ, Institut National de la Santé et de la Recherche Médicale, Service de Santé des Armées, Institut de Recherche Biomédicale des Armées, Membranes et Cibles Thérapeutiques, Marseille, France.

Jelena Pajovic (J)

DISCO Beamline, Synchrotron Soleil, Saint-Aubin, France.
University of Belgrade, Faculty of Physics, Belgrade, Serbia.

Satya P Bhamidimarri (SP)

Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

Koldo Morante (K)

Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

Jiajun Wang (J)

Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

Dmitrijs Lubriks (D)

Latvian Institute of Organic Synthesis, Riga, Latvia.

Edgars Suna (E)

Latvian Institute of Organic Synthesis, Riga, Latvia.

Robert A Stavenger (RA)

Antibacterial Discovery Performance Unit, Infectious Diseases Discovery, GlaxoSmithKline, Collegeville, PA, USA.

Mathias Winterhalter (M)

Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

Matthieu Réfrégiers (M)

DISCO Beamline, Synchrotron Soleil, Saint-Aubin, France.

Jean-Marie Pagès (JM)

Aix Marseille Univ, Institut National de la Santé et de la Recherche Médicale, Service de Santé des Armées, Institut de Recherche Biomédicale des Armées, Membranes et Cibles Thérapeutiques, Marseille, France.

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Classifications MeSH