In vitro gene expression and detergent-free reconstitution of active proteorhodopsin in lipid vesicles.

Detergents Gene Expression Lipids / chemistry Phosphatidylcholines / chemistry Protein Biosynthesis Rhodopsins, Microbial / metabolism Spectrometry, Fluorescence Transcription, Genetic Unilamellar Liposomes / chemistry
Lipids synthetic biology artificial cells cell-free protein expression protein reconstitution proteorhodopsin

Journal

Experimental biology and medicine (Maywood, N.J.)
ISSN: 1535-3699
Titre abrégé: Exp Biol Med (Maywood)
Pays: England
ID NLM: 100973463

Informations de publication

Date de publication:
03 2019
Historique:
pubmed: 12 1 2019
medline: 7 5 2020
entrez: 12 1 2019
Statut: ppublish

Résumé

Our results offer the potential for straightforward, additive-free, and molecularly simple routes to building complex bioreactors based on in vitro transcription-translation systems and lipid vesicles.

Identifiants

DOI: 10.1177/1535370218820290 PMID: 30630374 PMC: PMC6435890
pubmed: 30630374
doi: 10.1177/1535370218820290
pmc: PMC6435890
doi:

Substances chimiques

Detergents 0
Lipids 0
Phosphatidylcholines 0
Rhodopsins, Microbial 0
Unilamellar Liposomes 0
proteorhodopsin 0
1-palmitoyl-2-oleoylphosphatidylcholine TE895536Y5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

314-322

Références

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Auteurs

Giorgio Fracasso (G)

Max-Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

Yvonne Körner (Y)

Max-Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

David Thomas T Gonzales (DTT)

Max-Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

T-Y Dora Tang (TY)

Max-Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits domestiques › Détergents › In vitro gene expression and detergent-free...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › In vitro gene expression and detergent-free...
questionsmedicales.fr › Lipides › In vitro gene expression and detergent-free...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Glycérophosphate › Acides phosphatidiques › Glycérophospholipides › Phosphatidylcholines › In vitro gene expression and detergent-free...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › In vitro gene expression and detergent-free...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Photorécepteurs microbiens › Rhodopsines microbiennes › In vitro gene expression and detergent-free...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › In vitro gene expression and detergent-free...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › In vitro gene expression and detergent-free...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › Liposomes unilamellaires › In vitro gene expression and detergent-free...