STD NMR as a Technique for Ligand Screening and Structural Studies.
Binding epitope mapping
DEEP-STD NMR
Ligand screening
Protein–ligand interactions
STD NMR
Saturation transfer difference NMR
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
15
1
2019
pubmed:
15
1
2019
medline:
19
11
2019
Statut:
ppublish
Résumé
STD NMR is a powerful ligand-based tool for screening small molecules and low molecular weight fragments for their interaction with a given macromolecule. Such information is invaluable both in the drug discovery sector and in understanding fundamental biological interactions. Recently, powerful methods have been developed to extract a greater wealth of information from the STD NMR experiment, including ligand binding epitopes, dissociation constant determination, and mapping of binding site properties. Herein we describe these STD NMR experiments, giving practical examples for each approach, and highlight the important parameters and common pitfalls that must be considered for a successful experiment.
Identifiants
pubmed: 30638536
pii: S0076-6879(18)30296-9
doi: 10.1016/bs.mie.2018.08.018
pii:
doi:
Substances chimiques
Epitopes
0
Ligands
0
Macromolecular Substances
0
Proteins
0
Naproxen
57Y76R9ATQ
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
423-451Informations de copyright
© 2019 Elsevier Inc. All rights reserved.