β-Galactosidase from Exiguobacterium acetylicum: Cloning, expression, purification and characterization.
Exiguobacterium acetylicum
Lactose
Lactulose
β-Galactosidase
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
13
10
2018
revised:
31
12
2018
accepted:
02
01
2019
pubmed:
15
1
2019
medline:
17
8
2019
entrez:
15
1
2019
Statut:
ppublish
Résumé
The main goal of this work was to evaluate the performance of β-galactosidase from Exiguobacterium acetylicum MF03 in both hydrolysis and transgalactosylation reactions from different substrates. The enzyme gene was expressed in Escherichia coli BL21 (DE3), sequenced, and subjected to bioinformatic and kinetic assessment. Results showed that the enzyme was able to hydrolyze lactulose and o-nitrophenyl-β-d-galactopyranoside, but unable to hydrolyze lactose, o-nitrophenyl-β-d-glucopyranoside, butyl- and pentyl-β-d-galactosides. This unique and novel substrate specificity converts the E. acetylicum MF03 β-galactosidase into an ideal catalyst for the formulation of an enzymatic kit for lactulose quantification in thermally processed milk. This is because costly steps to eliminate glucose (resulting from hydrolysis of lactose when a customary β-galactosidase is used) can be avoided.
Identifiants
pubmed: 30639092
pii: S0960-8524(19)30008-2
doi: 10.1016/j.biortech.2019.01.005
pii:
doi:
Substances chimiques
beta-Galactosidase
EC 3.2.1.23
Types de publication
Journal Article
Langues
eng
Pagination
211-215Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.