Poly(azomethine-urethane) and zeolite-based composite: Fluorescent biosensor for DNA detection.
Animals
Azo Compounds
/ chemical synthesis
Biosensing Techniques
/ methods
Carbon-13 Magnetic Resonance Spectroscopy
Cattle
DNA
/ analysis
Fluorescent Dyes
/ chemistry
Hydrogen-Ion Concentration
Limit of Detection
Proton Magnetic Resonance Spectroscopy
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Thiosemicarbazones
/ chemical synthesis
Time Factors
Zeolites
/ chemistry
Biosensor
DNA
Fluorescent sensor
Zeolite
Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Titre abrégé: Spectrochim Acta A Mol Biomol Spectrosc
Pays: England
ID NLM: 9602533
Informations de publication
Date de publication:
05 Apr 2019
05 Apr 2019
Historique:
received:
10
08
2018
revised:
14
12
2018
accepted:
05
01
2019
pubmed:
15
1
2019
medline:
27
3
2019
entrez:
15
1
2019
Statut:
ppublish
Résumé
In the present paper, a highly selective and sensitive fluorescent biosensor based on poly(azomethine-urethane) and zeolite for the determination of DNA molecules was developed. Zeolite was chosen to enhance with anionic or cationic functional groups in polymer matrix and interaction between polymer and DNA. Several parameters such as polymer concentration, pH and incubation time effect on the sensitivity of the fluorescent biosensor were optimized. Linear range was determined between 2.50 and 25.00 nmol/L DNA concentration and limit of detection (LOD) of the biosensor was calculated as 0.095 nmol/L under the optimal conditions. Interference study was also performed in the presence of different amino acids, cations and organic compounds. The results clearly indicated that the tested cations and compounds were not induced a significant fluorescence change and the proposed zeolite-based biosensor was shown a good selectivity for DNA.
Identifiants
pubmed: 30641363
pii: S1386-1425(19)30011-3
doi: 10.1016/j.saa.2019.01.011
pii:
doi:
Substances chimiques
Azo Compounds
0
Fluorescent Dyes
0
Thiosemicarbazones
0
azomethine
0
poly(azomethine-urethane)
0
Zeolites
1318-02-1
DNA
9007-49-2
calf thymus DNA
91080-16-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
232-239Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.