Viral species richness and composition in young children with loose or watery stool in Ethiopia.
Diarrheal disease
Norwalk virus
Viral infection
Virome
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
14 Jan 2019
14 Jan 2019
Historique:
received:
30
04
2018
accepted:
02
01
2019
entrez:
16
1
2019
pubmed:
16
1
2019
medline:
6
3
2019
Statut:
epublish
Résumé
Stool consistency is an important diagnostic criterion in both research and clinical medicine and is often used to define diarrheal disease. We examine the pediatric enteric virome across stool consistencies to evaluate differences in richness and community composition using fecal samples collected from children aged 0 to 5 years participating in a clinical trial in the Amhara region of Ethiopia. The consistency of each sample was graded according to the modified Bristol Stool Form Scale for children (mBSFS-C) before a portion of stool was preserved for viral metagenomic analysis. Stool samples were grouped into 29 pools according to stool consistency type. Differential abundance was determined using negative-binomial modeling. Of 446 censused children who were eligible to participate, 317 presented for the study visit examination and 269 provided stool samples. The median age of children with stool samples was 36 months. Species richness was highest in watery-consistency stool and decreased as stool consistency became firmer (Spearman's r = - 0.45, p = 0.013). The greatest differential abundance comparing loose or watery to formed stool was for norovirus GII (7.64, 95% CI 5.8, 9.5) followed by aichivirus A (5.93, 95% CI 4.0, 7.89) and adeno-associated virus 2 (5.81, 95%CI 3.9, 7.7). In conclusion, we documented a difference in pediatric enteric viromes according to mBSFS-C stool consistency category, both in species richness and composition.
Sections du résumé
BACKGROUND
BACKGROUND
Stool consistency is an important diagnostic criterion in both research and clinical medicine and is often used to define diarrheal disease.
METHODS
METHODS
We examine the pediatric enteric virome across stool consistencies to evaluate differences in richness and community composition using fecal samples collected from children aged 0 to 5 years participating in a clinical trial in the Amhara region of Ethiopia. The consistency of each sample was graded according to the modified Bristol Stool Form Scale for children (mBSFS-C) before a portion of stool was preserved for viral metagenomic analysis. Stool samples were grouped into 29 pools according to stool consistency type. Differential abundance was determined using negative-binomial modeling.
RESULTS
RESULTS
Of 446 censused children who were eligible to participate, 317 presented for the study visit examination and 269 provided stool samples. The median age of children with stool samples was 36 months. Species richness was highest in watery-consistency stool and decreased as stool consistency became firmer (Spearman's r = - 0.45, p = 0.013). The greatest differential abundance comparing loose or watery to formed stool was for norovirus GII (7.64, 95% CI 5.8, 9.5) followed by aichivirus A (5.93, 95% CI 4.0, 7.89) and adeno-associated virus 2 (5.81, 95%CI 3.9, 7.7).
CONCLUSIONS
CONCLUSIONS
In conclusion, we documented a difference in pediatric enteric viromes according to mBSFS-C stool consistency category, both in species richness and composition.
Identifiants
pubmed: 30642268
doi: 10.1186/s12879-019-3674-3
pii: 10.1186/s12879-019-3674-3
pmc: PMC6332554
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
53Subventions
Organisme : National Institute of Child Health and Human Development
ID : F31 HD088070-01A1
Organisme : NEI NIH HHS
ID : U10 EY016214
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL105770
Pays : United States
Organisme : NEI NIH HHS
ID : U10 EY016214
Pays : United States
Organisme : NICHD NIH HHS
ID : F31 HD088070
Pays : United States
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