Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 01 2019
14 01 2019
Historique:
received:
22
06
2018
accepted:
06
11
2018
entrez:
16
1
2019
pubmed:
16
1
2019
medline:
30
1
2020
Statut:
epublish
Résumé
We developed a high-throughput mass spectrometry-based method to simultaneously quantify numerous small-molecule thiols and disulfides in blood plasma. Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment.
Identifiants
pubmed: 30643157
doi: 10.1038/s41598-018-35566-2
pii: 10.1038/s41598-018-35566-2
pmc: PMC6331564
doi:
Substances chimiques
Antioxidants
0
Biomarkers
0
Disulfides
0
Cysteine
K848JZ4886
Acetylcysteine
WYQ7N0BPYC
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
115Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL112633
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL117639
Pays : United States
Organisme : NHLBI NIH HHS
ID : R34 HL128798
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)
ID : HL112633
Pays : International
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