LAMP-based assay can rectify the diagnosis of Yersinia pseudotuberculosis infections otherwise missed by serology.
LAMP
Yersinia pseudotuberculosis
bacterial culture
serological testing
Journal
Journal of medical microbiology
ISSN: 1473-5644
Titre abrégé: J Med Microbiol
Pays: England
ID NLM: 0224131
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
pubmed:
17
1
2019
medline:
16
2
2019
entrez:
17
1
2019
Statut:
ppublish
Résumé
Despite being a well-known but seldom encountered zoonotic pathogen, diagnosis of Yersinia pseudotuberculosis is not necessarily easy. Infected patients occasionally present with various symptoms resembling Kawasaki disease; thus discriminating the two in the acute phase is challenging. In addition to bacterial culture and serology, novel detection methods based on loop-mediated isothermal amplification (LAMP) are reported in the literature. However, the clinical utility of LAMP-based methods in comparison with the other methods is scarcely documented in the literature. To clarify the clinical utility of a LAMP-based method in the diagnosis of Yersinia pseudotuberculosis infection. Inpatients admitted due to suspected Yersinia pseudotuberculosis infection during April 2008 through March 2015 were enrolled. Results of the LAMP-based method as well as culture and serology were collected and compared. Among 16 eligible cases, serology proved positive in 13 (81.3 %) cases, LAMP in eight (50 %) cases, and bacterial culture in four (25 %) cases. No significant difference among the three methods could be proved statistically. Although serology was the most sensitive method, it is known to miss cases such as young patients, whereas LAMP could complement all three cases missed by serology. Furthermore, LAMP can return the test result within a few hours from specimen receipt, whereas serology and bacterial culture requires days to weeks of time. Although second to serology in sensitivity, the LAMP-based method proved its utility in making rapid diagnosis, and serving a complementary role to serology.
Sections du résumé
BACKGROUND
BACKGROUND
Despite being a well-known but seldom encountered zoonotic pathogen, diagnosis of Yersinia pseudotuberculosis is not necessarily easy. Infected patients occasionally present with various symptoms resembling Kawasaki disease; thus discriminating the two in the acute phase is challenging. In addition to bacterial culture and serology, novel detection methods based on loop-mediated isothermal amplification (LAMP) are reported in the literature. However, the clinical utility of LAMP-based methods in comparison with the other methods is scarcely documented in the literature.
AIM
OBJECTIVE
To clarify the clinical utility of a LAMP-based method in the diagnosis of Yersinia pseudotuberculosis infection.
METHODOLOGY
METHODS
Inpatients admitted due to suspected Yersinia pseudotuberculosis infection during April 2008 through March 2015 were enrolled. Results of the LAMP-based method as well as culture and serology were collected and compared.
RESULTS
RESULTS
Among 16 eligible cases, serology proved positive in 13 (81.3 %) cases, LAMP in eight (50 %) cases, and bacterial culture in four (25 %) cases. No significant difference among the three methods could be proved statistically. Although serology was the most sensitive method, it is known to miss cases such as young patients, whereas LAMP could complement all three cases missed by serology. Furthermore, LAMP can return the test result within a few hours from specimen receipt, whereas serology and bacterial culture requires days to weeks of time.
CONCLUSION
CONCLUSIONS
Although second to serology in sensitivity, the LAMP-based method proved its utility in making rapid diagnosis, and serving a complementary role to serology.
Identifiants
pubmed: 30648936
doi: 10.1099/jmm.0.000868
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM