Noncovalently Associated Peptides Observed during Liquid Chromatography-Mass Spectrometry and Their Effect on Cross-Link Analyses.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
19 02 2019
19 02 2019
Historique:
pubmed:
17
1
2019
medline:
24
9
2020
entrez:
17
1
2019
Statut:
ppublish
Résumé
Cross-linking mass spectrometry draws structural information from covalently linked peptide pairs. When these links do not match to previous structural models, they may indicate changes in protein conformation. Unfortunately, such links can also be the result of experimental error or artifacts. Here, we describe the observation of noncovalently associated peptides during liquid chromatography-mass spectrometry analysis, which can easily be misidentified as cross-linked. Strikingly, they often mismatch to the protein structure. Noncovalently associated peptides presumably form during ionization and can be distinguished from cross-linked peptides by observing coelution of the corresponding linear peptides in MS1 spectra, as well as the presence of the individual (intact) peptide fragments in MS2 spectra. To suppress noncovalent peptide formations, increasingly disruptive ionization settings can be used, such as in-source fragmentation.
Identifiants
pubmed: 30649854
doi: 10.1021/acs.analchem.8b04037
pmc: PMC6396951
doi:
Substances chimiques
Cross-Linking Reagents
0
Myoglobin
0
Peptides
0
Conalbumin
1391-06-6
Creatine Kinase
EC 2.7.3.2
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2678-2685Subventions
Organisme : Wellcome Trust
ID : 203149
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108504
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 103139/Z/13/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 103139
Pays : United Kingdom
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