Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors.
Nuclear Proteins
/ genetics
Protein Serine-Threonine Kinases
/ genetics
RNA Processing, Post-Transcriptional
RNA, Fungal
/ genetics
RNA, Ribosomal
/ genetics
Ribosomal Proteins
/ genetics
Ribosome Subunits, Small, Eukaryotic
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
08
2018
accepted:
01
01
2019
entrez:
18
1
2019
pubmed:
18
1
2019
medline:
19
9
2019
Statut:
epublish
Résumé
Many of the small ribosomal subunit proteins are required for the stabilisation of late small ribosomal subunit (SSU) precursors and for final SSU rRNA processing in S. cerevisiae. Among them are ribosomal proteins (r-proteins) which form a protein cluster around rpS0 (uS2) at the "neck" of the SSU (S0-cluster) and others forming a nearby protein cluster around rpS3 (uS3) at the SSU "beak". Here we applied semi-quantitative proteomics together with complementary biochemical approaches to study how incomplete assembly of these two r-protein clusters affects binding and release of SSU maturation factors and assembly of other r-proteins in late SSU precursors in S. cerevisiae. For each of the two clusters specific impairment of the local r-protein assembly state was observed in Rio2 associated SSU precursors. Besides, cluster-specific effects on the association of biogenesis factors were detected. These suggested a role of S0-cluster formation for the efficient release of the two nuclear export factors Rrp12 and Slx9 from SSU precursors and for the correct incorporation of the late acting biogenesis factor Rio2. Based on our and on previous results we propose the existence of at least two different r-protein assembly checkpoints during late SSU maturation in S. cerevisiae. We discuss in the light of recent SSU precursor structure models how r-protein assembly states might be sensed by biogenesis factors at the S0-cluster checkpoint.
Identifiants
pubmed: 30653518
doi: 10.1371/journal.pone.0203415
pii: PONE-D-18-24306
pmc: PMC6336269
doi:
Substances chimiques
Nuclear Proteins
0
RNA, Fungal
0
RNA, Ribosomal
0
RPS3 protein, S cerevisiae
0
Ribosomal Proteins
0
SLX9 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
Rio2 protein, S cerevisiae
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0203415Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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