Human neutrophils express low levels of FcγRIIIA, which plays a role in PMN activation.


Journal

Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509

Informations de publication

Date de publication:
28 03 2019
Historique:
received: 19 07 2018
accepted: 09 01 2019
pubmed: 19 1 2019
medline: 4 12 2019
entrez: 19 1 2019
Statut: ppublish

Résumé

We have identified a rare healthy FcγRIIIB (CD16B)-null donor completely lacking FCGR3B RNA and protein expression and dissected the role of the different neutrophil Fcγ receptors in the response to therapeutic anti-CD20 monoclonal antibodies. We observed that polymorphonuclear neutrophils (PMNs) from FcγRIIIB wild-type (WT) individuals or the null donor were more effectively activated by chronic lymphocytic leukemia (CLL) B-cell targets opsonized with glycoengineered anti-CD20 antibodies compared with fully core-fucosylated anti-CD20 antibodies, suggesting the presence and role of FcγRIIIA (CD16A) on PMNs. Indeed, we demonstrated by reverse-transcription polymerase chain reaction, flow cytometry, and western blot analysis that PMNs from FcγRIIIB WT donors and the null individual express low levels of FcγRIIIA on their surfaces. FcγRIIIA is a functional and activating molecule on these cells, because anti-CD16 F(ab')

Identifiants

pubmed: 30655272
pii: S0006-4971(20)42662-X
doi: 10.1182/blood-2018-07-864538
pmc: PMC6484458
doi:

Substances chimiques

FCGR3A protein, human 0
Immunoglobulin G 0
RNA, Messenger 0
Receptors, IgG 0

Types de publication

Case Reports Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1395-1405

Commentaires et corrections

Type : CommentIn

Informations de copyright

© 2019 by The American Society of Hematology.

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Auteurs

Josée Golay (J)

Center of Cellular Therapy "G. Lanzani," Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy.
Fondazione per la Ricerca Ospedale Maggiore, Bergamo, Italy; and.

Rut Valgardsdottir (R)

Center of Cellular Therapy "G. Lanzani," Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy.

Gerta Musaraj (G)

Center of Cellular Therapy "G. Lanzani," Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy.

Damiano Giupponi (D)

Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy.

Orietta Spinelli (O)

Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy.

Martino Introna (M)

Center of Cellular Therapy "G. Lanzani," Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy.

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Classifications MeSH