Impaired butyrate absorption in the proximal colon, low serum butyrate and diminished central effects of butyrate on blood pressure in spontaneously hypertensive rats.
absorption
blood pressure
butyrate
gut microbiota
neuronal activity
Journal
Acta physiologica (Oxford, England)
ISSN: 1748-1716
Titre abrégé: Acta Physiol (Oxf)
Pays: England
ID NLM: 101262545
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
27
07
2018
revised:
03
12
2018
accepted:
11
01
2019
pubmed:
19
1
2019
medline:
22
7
2020
entrez:
19
1
2019
Statut:
ppublish
Résumé
Butyrate is a major gut microbiota-derived metabolite. Reduced butyrate-producing bacteria has been reported in the spontaneously hypertensive rat (SHR), a model of hypertension characterized by dysfunctional autonomic nervous system and gut dysbiosis. Here, we demonstrate a potential mechanism for butyrate in blood pressure regulation. High-performance liquid chromatography and liquid chromatography-mass spectrometry were performed to measure butyrate levels in feces and serum. Ussing chamber determined butyrate transport in colon ex vivo. Real-time PCR and immunohistochemistry evaluated expression of butyrate transporter, Slc5a8, in the colon. Mean arterial blood pressure was measured in catheterized anesthetized rats before and after a single butyrate intracerebroventricular injection. Activity of cardioregulatory brain regions was determined by functional magnetic resonance imaging to derive neural effects of butyrate. In the SHR, we demonstrated elevated butyrate levels in cecal content, but diminished butyrate levels in circulation, possibly due to reduced expression of Slc5a8 transporter in the colon. In addition, we observed lower expression levels of butyrate-sensing receptors in the hypothalamus of SHR, likely leading to the reduced effects of centrally administered butyrate on blood pressure in the SHR. Functional magnetic resonance imaging revealed reduced activation of cardioregulatory brain regions following central administration of butyrate in the SHR compared to control. We demonstrated a reduced availability of serum butyrate in the SHR, possibly due to diminished colonic absorption. Reduced expression of butyrate-sensing receptors in the SHR hypothalamus may explain the reduced central responsiveness to butyrate, indicating microbial butyrate may play a role in blood pressure regulation.
Identifiants
pubmed: 30656835
doi: 10.1111/apha.13256
pmc: PMC7199780
mid: NIHMS1065411
doi:
Substances chimiques
Butyrates
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13256Subventions
Organisme : NIH HHS
ID : OT2 OD023854
Pays : United States
Organisme : NHLBI NIH HHS
ID : R00 HL119560
Pays : United States
Organisme : American Heart Association
ID : 14SDG18300010
Pays : International
Organisme : UF CVM Start Up Funds
Pays : International
Informations de copyright
© 2019 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.
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