A "Clickable" Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
06 02 2019
06 02 2019
Historique:
pubmed:
19
1
2019
medline:
28
5
2020
entrez:
19
1
2019
Statut:
ppublish
Résumé
Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide-alkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules.
Identifiants
pubmed: 30657670
doi: 10.1021/jacs.8b13094
pmc: PMC6732186
mid: NIHMS1048438
doi:
Substances chimiques
Aza Compounds
0
Fluorescent Dyes
0
Small Molecule Libraries
0
alpha-Synuclein
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1893-1897Subventions
Organisme : NINDS NIH HHS
ID : R01 NS081033
Pays : United States
Organisme : NINDS NIH HHS
ID : R56 NS081033
Pays : United States
Organisme : NIA NIH HHS
ID : T32 AG000255
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118510
Pays : United States
Organisme : NINDS NIH HHS
ID : P50 NS053488
Pays : United States
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