Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan.
Chemo-enzymatic synthesis
Galactofuranose
Galactosidase
p-Nitrophenyl galactofuranoside
Journal
Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535
Informations de publication
Date de publication:
01 Feb 2019
01 Feb 2019
Historique:
received:
18
12
2018
revised:
10
01
2019
accepted:
10
01
2019
pubmed:
19
1
2019
medline:
6
5
2019
entrez:
19
1
2019
Statut:
ppublish
Résumé
β-d-Galactofuranose (Galf) is a component of polysaccharides and glycoconjugates. There are few reports about the involvement of galactofuranosyltransferases and galactofuranosidases (Galf-ases) in the synthesis and degradation of galactofuranose-containing glycans. The cell walls of filamentous fungi in the genus Aspergillus include galactofuranose-containing polysaccharides and glycoconjugates, such as O-glycans, N-glycans, and fungal-type galactomannan, which are important for cell wall integrity. In this study, we investigated the synthesis of p-nitrophenyl β-d-galactofuranoside and its disaccharides by chemo-enzymatic methods including use of galactosidase. The key step was selective removal of the concomitant pyranoside by enzymatic hydrolysis to purify p-nitrophenyl β-d-galactofuranoside, a promising substrate for β-d-galactofuranosidase from Streptomyces species.
Identifiants
pubmed: 30658252
pii: S0008-6215(18)30736-5
doi: 10.1016/j.carres.2019.01.005
pii:
doi:
Substances chimiques
Disaccharides
0
Mannans
0
galactomannan
11078-30-1
Galactosidases
EC 3.2.1.-
Galactose
X2RN3Q8DNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
99-103Informations de copyright
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