Poliovirus-binding inhibition ELISA based on specific chicken egg yolk antibodies as an alternative to the neutralization test.
Animals
Antibodies, Viral
/ blood
Chickens
/ immunology
Child, Preschool
Egg Yolk
/ immunology
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulins
/ immunology
Male
Neutralization Tests
Poliovirus
/ immunology
Poliovirus Vaccine, Inactivated
/ immunology
Poliovirus Vaccine, Oral
/ immunology
Virus Attachment
Binding inhibition ELISA
IgY
Neutralization test
Polio eradication
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
26
10
2018
revised:
14
01
2019
accepted:
15
01
2019
pubmed:
21
1
2019
medline:
7
3
2020
entrez:
21
1
2019
Statut:
ppublish
Résumé
The first application results of enzyme immunoassay system (ELISA) - binding inhibition ELISA (BI ELISA) for the detection of antibodies to polioviruses of three types based on the use of specific antibodies from chicken yolk (IgY) are presented. This variant of ELISA is a "surrogate" neutralization test (NT). When comparing the results of the detection of antibodies in 90 sera of children who were vaccinated with oral and inactivated poliovirus vaccines, good correlation (r = 0.67, 0.61, 0.76 for types 1, 2, 3 respectively) between the BI ELISA and the NT results was shown. The presented variant of ELISA is the further stage of the development of "IgY-technology" for laboratory diagnostics of viral infections, namely poliovirus infection, which can be used for seroepidemiological studies as an analogue of the NT which requires the use of life poliovirus with the required standard of biosafety containment facilities.
Identifiants
pubmed: 30660606
pii: S0166-0934(18)30531-7
doi: 10.1016/j.jviromet.2019.01.007
pii:
doi:
Substances chimiques
Antibodies, Viral
0
IgY
0
Immunoglobulins
0
Poliovirus Vaccine, Inactivated
0
Poliovirus Vaccine, Oral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7-10Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.