Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line.

Adolescent Apolipoproteins E / genetics Cell Culture Techniques / methods Cell Line Gene Editing Gene Knockout Techniques Homozygote Humans Induced Pluripotent Stem Cells / cytology Male Mutation / genetics

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
01 2019
Historique:
received: 15 06 2017
revised: 05 11 2018
accepted: 19 11 2018
pubmed: 21 1 2019
medline: 7 8 2019
entrez: 21 1 2019
Statut: ppublish

Résumé

Alzheimer's disease (AD) is the most frequent neurodegenerative disease amongst the elderly. The SNPs rs429358 and rs7412 in the APOE gene are the most common risk factor for sporadic AD, and there are three different alleles commonly referred to as APOE-ε2, APOE-ε3 and APOE-ε4. Induced pluripotent stem cells (iPSCs) hold great promise to model AD as such cells can be differentiated in vitro to the required cell type. Here we report the use of CRISPR/Cas9 technology employed on iPSCs from a healthy individual with an APOE-ε3/ε4 genotype to obtain isogenic APOE-ε2/ε2, APOE-ε3/ε3, APOE-ε4/ε4 lines as well as an APOE-knock-out line.

Identifiants

DOI: 10.1016/j.scr.2018.11.010 PMID: 30660866
pubmed: 30660866
pii: S1873-5061(18)30279-4
doi: 10.1016/j.scr.2018.11.010
pii:
doi:

Substances chimiques

Apolipoproteins E 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

101349

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Benjamin Schmid (B)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark. Electronic address: bsc@bioneer.dk.

Kennie R Prehn (KR)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Natakarn Nimsanor (N)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark; Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

Blanca Irene Aldana Garcia (BIA)

Neurometabolism Research Unit, Department of Drug Design and Pharmacology, University of Copenhagen, Denmark.

Ulla Poulsen (U)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Ida Jørring (I)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Mikkel A Rasmussen (MA)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Christian Clausen (C)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Ulrike A Mau-Holzmann (UA)

Institute of Medical Genetics and Applied Genomics, Division of Cytogenetics, Calwerstrasse 7, University of Tuebingen, 72076, Germany.

Sarayu Ramakrishna (S)

Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bengaluru, Karnataka, India; University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka, India.

Ravi Muddashetty (R)

Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bengaluru, Karnataka, India.

Rachel Steeg (R)

Censo Biotechnologies, Edinburgh EH25 9PP, UK.

Kevin Bruce (K)

Censo Biotechnologies, Edinburgh EH25 9PP, UK.

Peter Mackintosh (P)

Censo Biotechnologies, Edinburgh EH25 9PP, UK.

Andreas Ebneth (A)

Janssen Research & Development, Division of Janssen Pharmaceutica; N.V., Neuroscience Therapeutic Area, Turnhoutseweg 30, 2340 Beerse, Belgium.

Bjørn Holst (B)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Alfredo Cabrera-Socorro (A)

Janssen Research & Development, Division of Janssen Pharmaceutica; N.V., Neuroscience Therapeutic Area, Turnhoutseweg 30, 2340 Beerse, Belgium.

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Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adolescent › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lipoprotéines › Apolipoprotéines › Apolipoprotéines E › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-out de gènes › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Homozygote › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of a set of isogenic, gene-edited...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Generation of a set of isogenic, gene-edited...