Visualizing ubiquitination in mammalian cells.
LUBAC
OTULIN
Salmonella
super‐resolution microscopy
ubiquitination
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
01
06
2018
revised:
17
12
2018
accepted:
21
12
2018
pubmed:
23
1
2019
medline:
21
4
2020
entrez:
23
1
2019
Statut:
ppublish
Résumé
Covalent modification of proteins with ubiquitin is essential for the majority of biological processes in mammalian cells. Numerous proteins are conjugated with single or multiple ubiquitin molecules or chains in a dynamic fashion, often determining protein half-lives, localization or function. Experimental approaches to study ubiquitination have been dominated by genetic and biochemical analysis of enzyme structure-function relationships, reaction mechanisms and physiological relevance. Here, we provide an overview of recent developments in microscopy-based imaging of ubiquitination, available reagents and technologies. We discuss the progress in direct and indirect imaging of differentially linked ubiquitin chains in fixed and living cells using confocal fluorescence microscopy and super-resolution microscopy, illustrated by the role of ubiquitin in antibacterial autophagy and pro-inflammatory signalling. Finally, we speculate on future developments and forecast a transition from qualitative to quantitative super-resolution approaches to understand fundamental aspects of ubiquitination and the formation and distribution of functional E3 ligase protein complexes in their native environment.
Identifiants
pubmed: 30665942
pii: embr.201846520
doi: 10.15252/embr.201846520
pmc: PMC6362358
pii:
doi:
Substances chimiques
Ubiquitin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2019 The Authors.
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