Ultrathin Functional Polymer Modified Graphene for Enhanced Enzymatic Electrochemical Sensing.
Graphene
bio electrochemistry
biofunctionalization
electrochemical sensing
electropolymerization
enzyme immobilization
functional polymers
glucose biosensor
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
18 Jan 2019
18 Jan 2019
Historique:
received:
10
12
2018
revised:
11
01
2019
accepted:
15
01
2019
entrez:
24
1
2019
pubmed:
24
1
2019
medline:
30
4
2019
Statut:
epublish
Résumé
Grafting thin polymer layers on graphene enables coupling target biomolecules to graphene surfaces, especially through amide and aldehyde linkages with carboxylic acid and primary amine derivatives, respectively. However, functionalizing monolayer graphene with thin polymer layers without affecting their exceptional electrical properties remains challenging. Herein, we demonstrate the controlled modification of chemical vapor deposition (CVD) grown single layer graphene with ultrathin polymer 1,5-diaminonaphthalene (DAN) layers using the electropolymerization technique. It is observed that the controlled electropolymerization of DAN monomer offers continuous polymer layers with thickness ranging between 5⁻25 nm. The surface characteristics of pure and polymer modified graphene was examined. As anticipated, the number of surface amine groups increases with increases in the layer thickness. The effects of polymer thickness on the electron transfer rates were studied in detail and a simple route for the estimation of surface coverage of amine groups was demonstrated using the electrochemical analysis. The implications of grafting ultrathin polymer layers on graphene towards horseradish peroxidase (HRP) enzyme immobilization and enzymatic electrochemical sensing of H₂O₂ were discussed elaborately.
Identifiants
pubmed: 30669385
pii: bios9010016
doi: 10.3390/bios9010016
pmc: PMC6468408
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Polymers
0
Graphite
7782-42-5
Hydrogen Peroxide
BBX060AN9V
Horseradish Peroxidase
EC 1.11.1.-
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Engineering and Physical Sciences Research Council
ID : EP/M006301/1
Organisme : Innovate UK
ID : 102877
Organisme : European Regional Development Fund
ID : 663830-SU-077
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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