Purification and biochemical characterization of extracellular glucoamylase from Paenibacillus amylolyticus strain.
amylolyticus
characterization
glucoamylase
kinetics
purification
starch adsorption chromatography
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
27
09
2018
revised:
19
11
2018
accepted:
16
12
2018
pubmed:
27
1
2019
medline:
20
4
2019
entrez:
26
1
2019
Statut:
ppublish
Résumé
In the present study, glucoamylase produced from a soil bacterium Paenibacillus amylolyticus NEO03 was cultured under submerged fermentation conditions. The extracellular enzyme was purified by starch adsorption chromatography and further by gel filtration, with 2.73-fold and recovery of 40.02%. The protein exhibited molecular mass of ∼66,000 Da as estimated by SDS-PAGE and depicted to be a monomer. The enzyme demonstrated optimum activity at pH range 6.0-7.0 and temperature range 30-40 °C. Glucoamylase was mostly activated by Mn
Identifiants
pubmed: 30681161
doi: 10.1002/jobm.201800540
doi:
Substances chimiques
Culture Media
0
Starch
9005-25-8
Glucan 1,4-alpha-Glucosidase
EC 3.2.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
375-384Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.