Comparison of a chymase inhibitor and hyaluronic acid/carboxymethylcellulose (Seprafilm) in a novel peritoneal adhesion model in rats.
Animals
Carboxymethylcellulose Sodium
/ chemistry
Cecum
/ enzymology
Chymases
/ antagonists & inhibitors
Disease Models, Animal
Gene Expression
/ drug effects
Hyaluronic Acid
/ chemistry
Male
Peritoneal Diseases
/ chemically induced
Peroxidase
/ metabolism
Protease Inhibitors
/ pharmacology
Rats
Rats, Sprague-Dawley
Tissue Adhesives
/ pharmacology
Transforming Growth Factor beta
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
01
11
2018
accepted:
11
01
2019
entrez:
26
1
2019
pubmed:
27
1
2019
medline:
28
10
2019
Statut:
epublish
Résumé
Adhesion formation that occurred after alkali-induced injury of the cecum was used as a novel adhesion model in rats, and it was compared with that of a common adhesion model after abrading the cecum. Using the novel adhesion model, inhibition of adhesion formation by a chymase inhibitor, Suc-Val-Pro-PheP(OPh)2, and by sodium hyaluronate/carboxymethylcellulose (Seprafilm) was evaluated, and their mechanisms were assessed. The degree of adhesion formation was more severe and more stable in the alkali-induced injury model than in the abrasion-induced injury model. Both the chymase inhibitor and Seprafilm showed significant attenuation of the degree of adhesion 14 days after alkali-induced injury. Chymase activity in the cecum was significantly increased after alkali-induced injury, but it was significantly attenuated by the chymase inhibitor and Seprafilm. Myeloperoxidase and transforming-growth factor (TGF)-β levels were significantly increased after alkali-induced injury, but they were attenuated by both the chymase inhibitor and Seprafilm. At the level of the adhesions, the numbers of both chymase-positive cells and TGF-β-positive cells were significantly increased, but their numbers were reduced by the chymase inhibitor and Seprafilm. In conclusion, a chymase inhibitor attenuated the degree of adhesions to the same degree as Seprafilm in a novel peritoneal adhesion model that was more severe and more stable than the common adhesion model, and not only the chymase inhibitor, but also Seprafilm reduced the chymase increase at the adhesions.
Identifiants
pubmed: 30682159
doi: 10.1371/journal.pone.0211391
pii: PONE-D-18-31497
pmc: PMC6347210
doi:
Substances chimiques
Protease Inhibitors
0
Seprafilm
0
Tissue Adhesives
0
Transforming Growth Factor beta
0
Hyaluronic Acid
9004-61-9
Peroxidase
EC 1.11.1.7
Chymases
EC 3.4.21.39
Carboxymethylcellulose Sodium
K679OBS311
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0211391Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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