Differences in Self-Recognition between Secreted Antibody and Membrane-Bound B Cell Antigen Receptor.
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 03 2019
01 03 2019
Historique:
received:
16
05
2018
accepted:
20
12
2018
pubmed:
27
1
2019
medline:
13
11
2019
entrez:
27
1
2019
Statut:
ppublish
Résumé
The random gene segment rearrangement during B cell development ensures Ab repertoire diversity. Because this process might generate autoreactive specificities, it has been proposed that stringent selection mechanisms prevent the development of autoreactive B cells. However, conventional assays to identify autoreactive B cells usually employ in vitro-generated Abs, which differ from membrane-bound BCRs. In this study, we used a cell-based assay to investigate the autoreactivity of membrane-bound BCRs derived from different B cell developmental stages of human peripheral blood. Contrasted to soluble Ab counterparts, only a few of the tested BCRs were autoreactive, although the cell-based assay sensitively detects feeble Ag recognition of a germline-reverted murine BCR that was selected after OVA immunization of mice, whereas conventional assays failed to do so. Together, these data suggest that proper identification of autoreactive B cells requires the membrane-bound BCR, as the soluble Ab may largely differ from its BCR counterpart in Ag binding.
Identifiants
pubmed: 30683703
pii: jimmunol.1800690
doi: 10.4049/jimmunol.1800690
pmc: PMC6379807
doi:
Substances chimiques
Immunoglobulin M
0
Receptors, Antigen, B-Cell
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1417-1427Informations de copyright
Copyright © 2019 by The American Association of Immunologists, Inc.
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