Marine Microorganisms for Biocatalysis: Selective Hydrolysis of Nitriles with a Salt-Resistant Strain of Meyerozyma guilliermondii.
Biocatalysis
Marine yeast
Meyerozyma guilliermondii
Nitrilase
Seawater
Journal
Marine biotechnology (New York, N.Y.)
ISSN: 1436-2236
Titre abrégé: Mar Biotechnol (NY)
Pays: United States
ID NLM: 100892712
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
23
09
2018
accepted:
07
01
2019
pubmed:
27
1
2019
medline:
10
7
2019
entrez:
27
1
2019
Statut:
ppublish
Résumé
A screening among marine yeasts was carried out for nitrile hydrolyzing activity. Meyerozyma guilliermondii LM2 (UBOCC-A-214008) was able to efficiently grow on benzonitrile and cyclohexanecarbonitrile (CECN) as sole nitrogen sources. A two-step one-pot method for obtaining cells of M. guilliermondii LM2 (UBOCC-A-214008) endowed with high nitrilase activity was established; the resulting whole cells converted different nitriles with high molar conversions and showed interesting enantioselectivity toward racemic substrates. Nitrilase from M. guilliermondii LM2 (UBOCC-A-214008) displayed high activity on aromatic substrates, but also arylaliphatic and aliphatic substrates were accepted. Salt-resistant M. guilliermondii LM2 (UBOCC-A-214008) was used in media with different salinity, being highly active up to 1.5 M NaCl concentration. Finally, hydrolysis of nitriles was efficiently performed using a bioprocess (yeast growth and biotransformation with resting cells) entirely carried out in seawater.
Identifiants
pubmed: 30684102
doi: 10.1007/s10126-019-09875-0
pii: 10.1007/s10126-019-09875-0
doi:
Substances chimiques
Cyclohexanes
0
Nitriles
0
cyanocyclohexane
0
benzonitrile
9V9APP5H5S
Aminohydrolases
EC 3.5.4.-
nitrilase
EC 3.5.5.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
229-239Références
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