Characteristics of multicellular tumor spheroids formed by pancreatic cells expressing different adhesion molecules.
Antitumor drugs
Cadherin
Desmogleins
Multicellular tumor spheroids
Pancreatic cells
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Feb 2019
15 Feb 2019
Historique:
received:
29
10
2018
revised:
11
01
2019
accepted:
22
01
2019
pubmed:
27
1
2019
medline:
26
2
2019
entrez:
27
1
2019
Statut:
ppublish
Résumé
Multicellular tumor spheroids (MCTS) produced by different methods vary in forms, sizes, and properties. The aim of this work was to characterize MCTS formed by six pancreatic cell lines on a non-adherent surface. Human pancreatic cells were grown in 2D and 3D conditions and compared for the expression of E- and desmosomal cadherins (PCR, confocal microscopy), growth, cell cycling, apoptosis (flow cytometry), and a response to antitumor drugs doxorubicin and gemcitabine (MTT-assay). Three types of MCTS were identified: BxPC-3, T Taken collectively, pancreatic cells significantly vary in the expression of desmosomal cadherins, resulting in the formation of MCTS with different characteristics. The sensitivity of MCTS to various drugs depends on the type of cells and the method of spheroid preparation used.
Identifiants
pubmed: 30684543
pii: S0024-3205(19)30048-7
doi: 10.1016/j.lfs.2019.01.034
pii:
doi:
Substances chimiques
Antigens, CD
0
CDH1 protein, human
0
Cadherins
0
Desmosomal Cadherins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
343-352Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.