Is it possible to reconstruct an accurate cell lineage using CRISPR recorders?
CRISPR-based lineage recorders
D. melanogaster
cell lineage
computer simulation
developmental biology
zebrafish
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
28 01 2019
28 01 2019
Historique:
received:
13
08
2018
accepted:
11
01
2019
entrez:
29
1
2019
pubmed:
29
1
2019
medline:
9
4
2020
Statut:
epublish
Résumé
Cell lineages provide the framework for understanding how cell fates are decided during development. Describing cell lineages in most organisms is challenging; even a fruit fly larva has ~50,000 cells and a small mammal has >1 billion cells. Recently, the idea of applying CRISPR to induce mutations during development, to be used as heritable markers for lineage reconstruction, has been proposed by several groups. While an attractive idea, its practical value depends on the accuracy of the cell lineages that can be generated. Here, we use computer simulations to estimate the performance of these approaches under different conditions. We incorporate empirical data on CRISPR-induced mutation frequencies in This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).
Identifiants
pubmed: 30688650
doi: 10.7554/eLife.40292
pii: 40292
pmc: PMC6349403
doi:
pii:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Human Frontier Science Program
ID : HFSP RGP0002/2016
Pays : International
Informations de copyright
© 2019, Salvador-Martínez et al.
Déclaration de conflit d'intérêts
IS, MG, MA, MT No competing interests declared
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