Development of a Dual-Vector System Utilizing MicroRNA Mimics of the
Animals
Bacterial Proteins
/ genetics
Bacteriophage T7
/ enzymology
Baculoviridae
/ genetics
DNA-Directed RNA Polymerases
/ metabolism
Gene Knockdown Techniques
Genetic Engineering
Lepidoptera
/ genetics
Luminescent Proteins
/ genetics
MicroRNAs
/ genetics
Promoter Regions, Genetic
Recombinant Proteins
/ metabolism
Sf9 Cells
Viral Proteins
/ metabolism
RNA interference
artificial miRNA
baculovirus-insect cell expression system
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
27 Jan 2019
27 Jan 2019
Historique:
received:
07
01
2019
revised:
22
01
2019
accepted:
24
01
2019
entrez:
30
1
2019
pubmed:
30
1
2019
medline:
5
6
2019
Statut:
epublish
Résumé
The baculovirus-insect cell expression system is a popular tool for the manufacturing of various attractive recombinant products. Over the years, several attempts have been made to engineer and further improve this production platform by targeting host or baculoviral genes by RNA interference. In this study, an inducible knockdown system was established in insect (
Identifiants
pubmed: 30691228
pii: ijms20030533
doi: 10.3390/ijms20030533
pmc: PMC6387257
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Luminescent Proteins
0
MicroRNAs
0
Recombinant Proteins
0
Viral Proteins
0
yellow fluorescent protein, Bacteria
0
bacteriophage T7 RNA polymerase
EC 2.7.7.-
DNA-Directed RNA Polymerases
EC 2.7.7.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Austrian Science Fund FWF
ID : W 1224
Pays : Austria
Organisme : Federal Ministry of Science, Research and Economy (BMWFW), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol, the Government of Lower Austria and Business Agency Vienna
ID : COMET-Funding Program managed by the Austrian Research Promotion Agency FFG
Organisme : PhD programme "BioTop - Biomolecular Technology of Proteins"
ID : Austrian Science Funds, Project number FWF W1224
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