miR-4732-5p promotes breast cancer progression by targeting TSPAN13.
3' Untranslated Regions
Adult
Aged
Aged, 80 and over
Antagomirs
/ genetics
Apoptosis
/ genetics
Base Sequence
Breast Neoplasms
/ genetics
Cell Line, Tumor
Cell Movement
Cell Proliferation
Disease Progression
Female
Gene Expression Regulation, Neoplastic
Humans
Lymphatic Metastasis
MicroRNAs
/ agonists
Middle Aged
Neoplasm Staging
Oligoribonucleotides
/ genetics
Signal Transduction
Survival Analysis
Tetraspanins
/ genetics
TSPAN13
breast cancer
metastasis
miR-4732-5p
proliferation
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
21
08
2018
revised:
22
11
2018
accepted:
17
12
2018
pubmed:
1
2
2019
medline:
16
7
2020
entrez:
1
2
2019
Statut:
ppublish
Résumé
MiR-4732-5p was previously found to be dysregulated in nipple discharge of breast cancer. However, the expression and function of miR-4732-5p in breast cancer remain largely unknown. Here, the expression of miR-4732-5p was detected using quantitative real-time PCR in breast cancer tissues and cell lines. Cell proliferation, apoptosis, migration and invasion assays were performed to examine the effects of miR-4732-5p in breast cancer. In addition, mRNA sequencing, bioinformatics analysis, Western blot and luciferase assays were performed to identify the target of miR-4732-5p. Overall, miR-4732-5p was significantly down-regulated in breast cancer tissues, especially in lymph node metastasis (LNM)-negative tissues, compared with adjacent normal tissues. However, it was more highly expressed in LNM-positive breast cancer tissues, compared with LNM-negative ones. Expression of miR-4732-5p was positively correlated with lymph node metastasis, larger tumour size, advanced clinical stage, high Ki-67 levels and poor prognosis. MiR-4732-5p promoted cell proliferation, migration and invasion in breast cancer. MiR-4732-5p directly targeted the 3'-UTR of tetraspanin 13 (TSPAN13) and suppressed TSPAN13 expression at the mRNA and protein levels. These results suggested that miR-4732-5p may serve as a tumour suppressor in the initiation of breast cancer, but as a tumour promoter in breast cancer progression by targeting TSPAN13.
Identifiants
pubmed: 30701690
doi: 10.1111/jcmm.14145
pmc: PMC6433729
doi:
Substances chimiques
3' Untranslated Regions
0
Antagomirs
0
MicroRNAs
0
Oligoribonucleotides
0
TSPAN13 protein, human
0
Tetraspanins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2549-2557Informations de copyright
© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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