The production of ethanol from lignocellulosic biomass by Kluyveromyces marxianus CICC 1727-5 and Spathaspora passalidarum ATCC MYA-4345.


Journal

Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612

Informations de publication

Date de publication:
Mar 2019
Historique:
received: 02 09 2018
accepted: 16 12 2018
revised: 18 11 2018
pubmed: 2 2 2019
medline: 19 7 2019
entrez: 2 2 2019
Statut: ppublish

Résumé

Efficient bioconversion of lignocellulosic biomass is one of the key challenges for the production of bioethanol and chemicals. Therefore, the present work focuses on finding a robust microorganism able to convert all sugars in lignocellulosic hydrolysates efficiently. The fermentation performance showed that Kluyveromyces marxianus CICC 1727-5 could produce ethanol from glucose with productivity 4.2 g/L/h and higher ethanol yields (0.44 g/g) under 40 °C, outdistance the productivity 0.258 g/L/h of S. passalidarum ATCC MYA-4345. The xylose utilization of S. passalidarum ATCC MYA-4345 was faster than K. marxianus CICC 1727-5 with the ethanol yield 0.31 g/g at 30 °C. However, K. marxianus CICC 1727-5 could produce xylitol from xylose with the yield 0.58 g/g at 40 °C. Meanwhile, the two yeasts both had the ability to use arabinose naturally, but K. marxianus CICC 1727-5 could consume arabinose completely and quickly. Furthermore, the two yeasts both could ferment glucose and xylose simultaneously, but K. marxianus CICC 1727-5 showed much better performance in the cofermentation. The peak ethanol concentration of K. marxianus CICC 1727-5 and S. passalidarum ATCC MYA-4345 was 42.6 and 31.9 g/L, respectively. In the saccharification and cofermentation (SSCF) process using non-detoxificated corncob, K. marxianus CICC 1727-5 showed better performance. K. marxianus CICC 1727-5 was more tolerant in the presence of formic acid, acetic acid, and mix inhibitors and even was capable to grow in the medium with the acetic acid concentration up to 15 g/L. K. marxianus CICC 1727-5 is a promising candidate strain for further metabolic engineering to develop robust industrial strains for the lignocellulosic ethanol.

Identifiants

pubmed: 30706114
doi: 10.1007/s00253-019-09625-1
pii: 10.1007/s00253-019-09625-1
doi:

Substances chimiques

Biofuels 0
Sugars 0
lignocellulose 11132-73-3
Ethanol 3K9958V90M
Lignin 9005-53-2
Xylose A1TA934AKO
Arabinose B40ROO395Z
Xylitol VCQ006KQ1E

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2845-2855

Subventions

Organisme : Postdoctoral Research Foundation of China
ID : 2015M571316

Auteurs

Cong Du (C)

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.

Yinmin Li (Y)

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.

Xiaoyang Zhao (X)

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.

Xuze Pei (X)

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China.

Wenjie Yuan (W)

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China. ywj@dlut.edu.cn.

Fengwu Bai (F)

State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China.

Yu Jiang (Y)

Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, 15261, USA.

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Classifications MeSH