Effects of topical methotrexate loaded gold nanoparticle in cutaneous inflammatory mouse model.
Administration, Topical
Animals
Disease Models, Animal
Drug Carriers
/ chemistry
Gold
/ chemistry
Immunosuppressive Agents
/ administration & dosage
Metal Nanoparticles
/ chemistry
Methotrexate
/ administration & dosage
Mice
Mice, Inbred C57BL
Psoriasis
/ drug therapy
Sulfhydryl Compounds
/ chemistry
Functionalized gold nanoparticle
Methotrexate transepidermal delivery
Percutaneous delivery
Topical psoriasis treatment
Journal
Nanomedicine : nanotechnology, biology, and medicine
ISSN: 1549-9642
Titre abrégé: Nanomedicine
Pays: United States
ID NLM: 101233142
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
04
07
2018
revised:
14
01
2019
accepted:
15
01
2019
pubmed:
2
2
2019
medline:
22
11
2019
entrez:
2
2
2019
Statut:
ppublish
Résumé
Gold nanoparticles functionalized with 3-mercapto-1-propansulfonate (AuNPs-3MPS) have been prepared and loaded with Methotrexate (MTX), an immunosuppressive agent used in the systemic treatment of moderate-severe inflammatory diseases. The effects of the AuNPs-3MPS@MTX topically administered in vitro on skin model and in vivo on imiquimod-induced psoriasis-like mice model, have been studied. Clinical response, epidermal thickness, cell proliferation rate and inflammation were tested. AuNPs-3MPS@MTX treated mice showed a decreasing of scaling and erythema score, reduction of epidermal thickness, parakeratosis and hyperkeratosis, compared to AuNPs-3MPS treated mice. Immunohistochemistry analysis staining displayed that Ki67, K6 CD3 and CD8 stainings were reduced in AuNPs-3MPS@MTX treated mice. Blood evaluation showed no differences in blood count and in ALT and AST levels before and after AuNPs-3MPS or AuNPs-3MPS@MTX treatment. Topical AuNPs-3MPS@MTX treatment is able to induce a reduction of keratinocytes hyperproliferation, epidermal thickness and also inflammatory infiltrate in vivo on imiquimod-induced psoriasis like mice model.
Identifiants
pubmed: 30708054
pii: S1549-9634(19)30018-8
doi: 10.1016/j.nano.2019.01.006
pii:
doi:
Substances chimiques
3-mercapto-1-propanesulfonate
0
Drug Carriers
0
Immunosuppressive Agents
0
Sulfhydryl Compounds
0
Gold
7440-57-5
Methotrexate
YL5FZ2Y5U1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
276-286Informations de copyright
Published by Elsevier Inc.