Investigation of multidrug-resistant ST2 Acinetobacter baumannii isolated from Saint George hospital in Lebanon.
Acinetobacter Infections
/ epidemiology
Acinetobacter baumannii
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Bacterial Typing Techniques
Cross Infection
/ epidemiology
DNA, Bacterial
/ genetics
Drug Resistance, Multiple, Bacterial
/ genetics
Female
Hospitals
Humans
Lebanon
/ epidemiology
Male
Microbial Sensitivity Tests
Multilocus Sequence Typing
Pneumonia, Ventilator-Associated
/ microbiology
Sputum
/ microbiology
Acinetobacter baumannii
OXA-23
ST2 clone
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
02 02 2019
02 02 2019
Historique:
received:
25
07
2018
accepted:
24
01
2019
entrez:
4
2
2019
pubmed:
4
2
2019
medline:
18
12
2019
Statut:
epublish
Résumé
Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase bla This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having bla
Sections du résumé
BACKGROUND
Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon.
METHODS
Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis.
RESULTS
Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D carbapenemase bla
CONCLUSIONS
This study shows a nosocomial spread of multidrug-resistant A. baumannii ST2 having bla
Identifiants
pubmed: 30710998
doi: 10.1186/s12866-019-1401-2
pii: 10.1186/s12866-019-1401-2
pmc: PMC6359860
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA, Bacterial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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