Highly-branched poly(N-isopropyl acrylamide) functionalised with pendant Nile red and chain end vancomycin for the detection of Gram-positive bacteria.
Bacterial sensor
Diagnostic device
Polymer architecture
Solvatochromism
Specificity
Stimuli responsive
Journal
Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144
Informations de publication
Date de publication:
15 03 2019
15 03 2019
Historique:
received:
20
10
2018
revised:
27
01
2019
accepted:
30
01
2019
pubmed:
4
2
2019
medline:
9
4
2020
entrez:
4
2
2019
Statut:
ppublish
Résumé
This study shows how highly branched poly(N-isopropyl acrylamide) (HB-PNIPAM) with a chain pendant solvatochromic dye (Nile red) could provide a fluorescence signal, as end groups bind to bacteria and chain segments become desolvated, indicating the presence of bacteria. Vancomycin was attached to chain ends of HB-PNIPAM or as pendant groups on linear polymers each containing Nile red. Location of the dye was varied between placement in the core of the branched polymer coil or the outer domains. Both calorimetric and fluorescence data showed that branched polymers responded to binding of both the peptide target (D-Ala-D-Aa) and bacteria in a different manner than analogous linear polymers; binding and response was more extensive in the branched variant. The fluorescence data showed that only segments located in the outer domains of branched polymers responded to binding of Gram-positive bacteria with little response when linear analogous polymer or branched polymer with the dye in the inner core was exposed to Staphylococcus aureus.
Identifiants
pubmed: 30711663
pii: S1742-7061(19)30088-1
doi: 10.1016/j.actbio.2019.01.066
pmc: PMC6401204
pii:
doi:
Substances chimiques
Acrylic Resins
0
poly-N-isopropylacrylamide
25189-55-3
Vancomycin
6Q205EH1VU
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
197-206Subventions
Organisme : Medical Research Council
ID : MR/N501888/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N501888/2
Pays : United Kingdom
Informations de copyright
Copyright © 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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