Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs.
Cardiovascular Diseases
/ drug therapy
Cell Adhesion
Cytokines
/ metabolism
DNA, Complementary
/ metabolism
Dose-Response Relationship, Radiation
Endothelium, Vascular
/ pathology
Human Umbilical Vein Endothelial Cells
/ radiation effects
Humans
Inflammation
/ drug therapy
MicroRNAs
/ metabolism
Vascular Cell Adhesion Molecule-1
/ metabolism
2 Gy
HUVECs
IL8
Radiation
human umbilical vein endothelial cells
interleukin 8
miRNA
sVCAM1
soluble vascular cell adhesion molecule 1
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
12
12
2018
revised:
21
01
2019
accepted:
22
01
2019
entrez:
4
2
2019
pubmed:
4
2
2019
medline:
21
3
2019
Statut:
ppublish
Résumé
Radiotherapy (RT) can lead to cardiovascular disease (CVD). Evidence suggests that radiation modulates miRNA levels. Our purpose was to assess the acute response to radiation-induced modulation of the expression of miRNA-146a, miRNA-155, miRNA-221, and miRNA-222, inflammatory response and endothelial dysfunction on endothelial cells. Human umbilical vein endothelial cells (HUVECs) were exposed to 2 Gy RT, and intracellular levels of selected miRNAs were measured by real-time polymerase chain reaction at 2 and 24 h. Cytokine and adhesion molecule release were also assessed. Results showed that 2 Gy significantly increased the expression of miRNA-221 and miRNA-222, and reduced the level of miRNA-155 after 2 h; whereas miRNA-146a and miRNA-155 were significantly overexpressed and miRNA-222 was significantly down-regulated at 24 h. Interleukin-8 and soluble vascular cell adhesion molecule 1 levels were not affected by the studied RT. RT at 2 Gy modulated expression of selected miRNAs by endothelial cells after 2 and 24 h, which might be related to CVD development in patients who receive RT.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Radiotherapy (RT) can lead to cardiovascular disease (CVD). Evidence suggests that radiation modulates miRNA levels. Our purpose was to assess the acute response to radiation-induced modulation of the expression of miRNA-146a, miRNA-155, miRNA-221, and miRNA-222, inflammatory response and endothelial dysfunction on endothelial cells.
MATERIALS AND METHODS
METHODS
Human umbilical vein endothelial cells (HUVECs) were exposed to 2 Gy RT, and intracellular levels of selected miRNAs were measured by real-time polymerase chain reaction at 2 and 24 h. Cytokine and adhesion molecule release were also assessed.
RESULTS
RESULTS
Results showed that 2 Gy significantly increased the expression of miRNA-221 and miRNA-222, and reduced the level of miRNA-155 after 2 h; whereas miRNA-146a and miRNA-155 were significantly overexpressed and miRNA-222 was significantly down-regulated at 24 h. Interleukin-8 and soluble vascular cell adhesion molecule 1 levels were not affected by the studied RT.
CONCLUSION
CONCLUSIONS
RT at 2 Gy modulated expression of selected miRNAs by endothelial cells after 2 and 24 h, which might be related to CVD development in patients who receive RT.
Identifiants
pubmed: 30711956
pii: 39/2/771
doi: 10.21873/anticanres.13174
doi:
Substances chimiques
Cytokines
0
DNA, Complementary
0
MIRN146 microRNA, human
0
MIRN155 microRNA, human
0
MIRN221 microRNA, human
0
MIRN222 microRNA, human
0
MicroRNAs
0
Vascular Cell Adhesion Molecule-1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
771-780Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.