Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract.
Animals
Cell Differentiation
/ genetics
Cell Line
Cells, Cultured
Embryonic Stem Cells
/ metabolism
Endothelial Cells
/ metabolism
Heart Ventricles
/ metabolism
Human Embryonic Stem Cells
/ metabolism
Humans
LIM-Homeodomain Proteins
/ genetics
Mice, Inbred C57BL
Multipotent Stem Cells
Myocardium
/ metabolism
Myocytes, Cardiac
/ metabolism
Organogenesis
Receptors, G-Protein-Coupled
/ metabolism
Single-Cell Analysis
/ methods
Wnt signaling
cardiac development
cardiac progenitor cell
embryogenesis
pluripotent stem cell
single-cell RNA-seq
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
25 02 2019
25 02 2019
Historique:
received:
07
09
2018
revised:
18
12
2018
accepted:
31
12
2018
pubmed:
5
2
2019
medline:
23
8
2019
entrez:
5
2
2019
Statut:
ppublish
Résumé
The morphogenetic process of mammalian cardiac development is complex and highly regulated spatiotemporally by multipotent cardiac stem/progenitor cells (CPCs). Mouse studies have been informative for understanding mammalian cardiogenesis; however, similar insights have been poorly established in humans. Here, we report comprehensive gene expression profiles of human cardiac derivatives from multipotent CPCs to intermediates and mature cardiac cells by population and single-cell RNA-seq using human embryonic stem cell-derived and embryonic/fetal heart-derived cardiac cells micro-dissected from specific heart compartments. Importantly, we discover a uniquely human subset of cono-ventricular region-specific CPCs, marked by LGR5. At 4 to 5 weeks of fetal age, the LGR5
Identifiants
pubmed: 30713072
pii: S1534-5807(19)30005-X
doi: 10.1016/j.devcel.2019.01.005
pii:
doi:
Substances chimiques
LGR5 protein, human
0
LIM-Homeodomain Proteins
0
Lgr5 protein, mouse
0
Receptors, G-Protein-Coupled
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
475-490.e7Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.