Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan.


Journal

The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360

Informations de publication

Date de publication:
30 Mar 2019
Historique:
pubmed: 5 2 2019
medline: 23 5 2019
entrez: 5 2 2019
Statut: ppublish

Résumé

Six atypical Actinobacillus pleuropneumoniae serovar 15 strains were isolated from pneumonic lesions of naturally infected dead pigs from the same farm in Japan. Genetic analyses of apx genes revealed that the atypical isolates contained the toxin-associated genes apxIBD, apxIIICA, apxIIIBD, and apxIVA, but not apxIICA. Coinciding with the result of the atypical gene profile, analyses of toxin protein production revealed that these atypical isolates expressed only ApxIII but not ApxII. A mouse pathogenicity test showed that the atypical isolate tested seemed to be less virulent than the typical isolates. This is the first report describing the emergence of atypical A. pleuropneumoniae serovar 15, which does not produce ApxII due to the absence of apxIICA genes, in Japan.

Identifiants

pubmed: 30713216
doi: 10.1292/jvms.18-0421
pmc: PMC6451906
doi:

Substances chimiques

ApxII toxin, bacteria 0
Bacterial Proteins 0
Hemolysin Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

480-485

Références

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Auteurs

Kaho Teshima (K)

Nippon Institute for Biological Science, Tokyo 198-0024, Japan.

Haruna Hirano (H)

Yamanashi Prefecture Eastern Livestock Hygiene Service Center, 1001-1 Karakashiwa, Ishawa, Fuefuki, Yamanashi 406-0034, Japan.

Kazutada Ushiyama (K)

Yamanashi Prefecture Eastern Livestock Hygiene Service Center, 1001-1 Karakashiwa, Ishawa, Fuefuki, Yamanashi 406-0034, Japan.

Kazumoto Shibuya (K)

Nippon Institute for Biological Science, Tokyo 198-0024, Japan.

Shinya Nagai (S)

Nippon Institute for Biological Science, Tokyo 198-0024, Japan.

Chihiro Sasakawa (C)

Nippon Institute for Biological Science, Tokyo 198-0024, Japan.
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chiba 260-8637, Japan.

Ho To (H)

Nippon Institute for Biological Science, Tokyo 198-0024, Japan.

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Classifications MeSH