3-dimensional visualization of implant-tissue interface with the polyethylene glycol associated solvent system tissue clearing method.
Animals
Bone-Implant Interface
/ anatomy & histology
Decalcification Technique
Dental Implants
Histocytological Preparation Techniques
/ methods
Humans
Imaging, Three-Dimensional
Mandible
/ anatomy & histology
Mice
Mice, Transgenic
Microscopy, Fluorescence, Multiphoton
Neovascularization, Physiologic
Osseointegration
Polyethylene Glycols
Solvents
Stainless Steel
Titanium
PEGASOS
angiogenesis
implant
osseointegration
tissue clearing
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
07
12
2018
revised:
14
12
2018
accepted:
28
12
2018
pubmed:
5
2
2019
medline:
18
6
2019
entrez:
5
2
2019
Statut:
ppublish
Résumé
Dental implants are major treatment options for restoring teeth loss. Biological processes at the implant-tissue interface are critical for implant osseointegration. Superior mechanical properties of the implant constitute a major challenge for traditional histological techniques. It is imperative to develop new technique to investigate the implant-tissue interface. Our laboratory developed the polyethylene glycol (PEG)-associated solvent system (PEGASOS) tissue clearing method. By immersing samples into various chemical substances, bones and teeth could be turned to transparent with intact internal structures and endogenous fluorescence being preserved. We combined the PEGASOS tissue clearing method with transgenic mouse line and other labelling technique to investigate the angiogenesis and osteogenesis processes occurring at the implant-bone interface. Clearing treatment turned tissue highly transparent and implant could be directly visualized without sectioning. Implant, soft/hard tissues and fluorescent labels were simultaneously imaged in decalcified or non-decalcified mouse mandible samples without disturbing their interfaces. Multi-channel 3-dimensional image stacks at high resolution were acquired and quantified. The processes of angiogenesis and osteogenesis surrounding titanium or stainless steel implants were investigated. Both titanium and stainless steel implants support angiogenesis at comparable levels. Successful osseointegration and calcium precipitation occurred only surrounding titanium, but not stainless steel implants. PEGASOS tissue clearing method provides a novel approach for investigating the interface between implants and hard tissue.
Identifiants
pubmed: 30714253
doi: 10.1111/cpr.12578
pmc: PMC6536405
mid: NIHMS1005751
doi:
Substances chimiques
Dental Implants
0
Solvents
0
Stainless Steel
12597-68-1
Polyethylene Glycols
3WJQ0SDW1A
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12578Subventions
Organisme : National Institute of Dental and Craniofacial Research
ID : R21 DE027928
Organisme : American Heart Association
ID : 17IRG33410377
Organisme : NINDS NIH HHS
ID : R21 NS099950
Pays : United States
Organisme : NIDCR NIH HHS
ID : R21 DE027928
Pays : United States
Organisme : National Natural Science Foundation of China
ID : 81470776
Organisme : National Institute of Dental and Craniofacial Research
ID : K08DE025090
Organisme : National Institute of Neurological Disorders and Stroke
ID : R00NS073735
Organisme : NIDCR NIH HHS
ID : K08 DE025090
Pays : United States
Organisme : National Institute of Neurological Disorders and Stroke
ID : 5R21NS099950
Organisme : NINDS NIH HHS
ID : R00 NS073735
Pays : United States
Informations de copyright
© 2019 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.
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