Evaluation of the effect of teriparatide therapy on mandibular fracture healing in rats with medication-related osteonecrosis of the jaw.


Journal

Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 11 09 2017
accepted: 25 01 2019
pubmed: 5 2 2019
medline: 7 1 2020
entrez: 5 2 2019
Statut: ppublish

Résumé

The aim of this study was to evaluate the effect of teriparatide therapy on mandibular fracture healing in rats with medication-related osteonecrosis of the jaw (MRONJ). To induce MRONJ, a total of 120 rats received intravenous zoledronate 0.06 mg/kg once a week for 6 weeks and their right mandibular first molar was extracted. Eighty of 94 rats with MRONJ were randomly selected and underwent unilateral mandibular osteotomy to replicate a fracture. After surgery, the rats were randomly assigned to T (teriparatide-treated) and C (control) groups. Group T (n = 40) received subcutaneous injection of 2 μg/kg/day teriparatide and group C (n = 40) received the same volume of normal saline until sacrifice. Four and 8 weeks after surgery, 20 rats in each group were sacrificed. Fracture healing was scored using a histological grading system (1 to 10). In group C, at 4 weeks and 8 weeks post-fracture, fibrous and cartilaginous tissues and scant bone formation at the fracture site and lacunae without osteocyte in adjacent mandibular bone were seen. In group T, substantial amounts of new trabecular bone rimmed by osteoblasts and some areas of remodeled mature bone were seen. After 8 weeks, extensive replacement of trabecular bone with mature bone occurred. Except between C4 and C8 groups, the healing score was significantly different between all subgroups. Teriparatide therapy successfully improved mandibular fracture healing in rats with MRONJ. However, this study was limited by the use of an animal model whose anatomy, physiology, and drug metabolism might be different from humans. The present study showed that teriparatide therapy may be used adjunctive to surgery in the treatment of mandibular fractures in MRONJ patients.

Identifiants

pubmed: 30715621
doi: 10.1007/s00784-019-02830-2
pii: 10.1007/s00784-019-02830-2
doi:

Substances chimiques

Bone Density Conservation Agents 0
Teriparatide 10T9CSU89I

Types de publication

Journal Article

Langues

eng

Pagination

3987-3993

Subventions

Organisme : Hamedan University of Medical Sciences
ID : 9507134134

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Auteurs

Mohammad Zandi (M)

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hamadan University of Medical Sciences, Shahid Fahmideh Street, Hamadan, Iran. zandi88m@yahoo.com.
Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. zandi88m@yahoo.com.

Arash Dehghan (A)

Department of Pathology, Hamadan University of Medical Sciences, Hamadan, Iran.

Payam Amini (P)

Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Shideh Doulati (S)

Department of Oral and Maxillofacial Surgery, Hamadan University of Medical Sciences, Hamadan, Iran.

Leila Rezaeian (L)

Department of Oral and Maxillofacial Surgery, Hamadan University of Medical Sciences, Hamadan, Iran.

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Classifications MeSH