Inducing a Mucosal Barrier-Sparing Inflammatory Response in Laboratory-Grown Primary Human Nasal Epithelial Cells.

Cells, Cultured Epithelial Cells / drug effects Guidelines as Topic Humans Interleukin-6 / biosynthesis Nasal Mucosa / cytology Poly I-C / pharmacology Primary Cell Culture / methods Toll-Like Receptor 3 / agonists
IL-6 TLR 3 air-liquid interface cultures chronic rhinosinusitis immunofluorescence poly (I:C) LMW primary human nasal epithelial cells

Journal

Current protocols in toxicology
ISSN: 1934-9262
Titre abrégé: Curr Protoc Toxicol
Pays: United States
ID NLM: 9816330

Informations de publication

Date de publication:
06 2019
Historique:
pubmed: 5 2 2019
medline: 21 4 2020
entrez: 5 2 2019
Statut: ppublish

Résumé

Here we use the toll-like receptor (TLR) 3 agonist poly I:C (LMW) to induce an inflammatory response in cells of submerged and/or air-liquid interface (ALI) cultures of human nasal epithelial cells (HNECs). The inflammatory response is determined by measuring interleukin-6 (IL-6) protein levels by enzyme-linked immunosorbent assay (ELISA). The mucosal barrier integrity is determined by measuring transepithelial electrical resistance (TEER) and passage of fluorescently labeled dextrans. Stimulation with poly (I:C) LMW induces a 15- to 17-fold increase in IL-6 production by HNEC-ALI cells. © 2019 by John Wiley & Sons, Inc.

Identifiants

DOI: 10.1002/cptx.69 PMID: 30715797
pubmed: 30715797
doi: 10.1002/cptx.69
doi:

Substances chimiques

IL6 protein, human 0
Interleukin-6 0
TLR3 protein, human 0
Toll-Like Receptor 3 0
Poly I-C O84C90HH2L

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e69

Informations de copyright

© 2019 John Wiley & Sons, Inc.

Auteurs

Mahnaz Ramezanpour (M)

Department of Surgery, Otorhinolaryngology Head and Neck Surgery, Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia, Australia.

Harrison Bolt (H)

Department of Surgery, Otorhinolaryngology Head and Neck Surgery, Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia, Australia.

Alkis Psaltis (A)

Department of Surgery, Otorhinolaryngology Head and Neck Surgery, Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia, Australia.

Peter-John Wormald (PJ)

Department of Surgery, Otorhinolaryngology Head and Neck Surgery, Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia, Australia.

Sarah Vreugde (S)

Department of Surgery, Otorhinolaryngology Head and Neck Surgery, Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia, Australia.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Cellules › Cellules épithéliales › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Qualité, accès, évaluation des soins de santé › Assurance de la qualité des soins de santé › Recommandations comme sujet › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interleukines › Interleukine-6 › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Tissus › Membranes › Muqueuse › Muqueuse respiratoire › Muqueuse nasale › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Polyribonucléotides › Poly I › Poly I-C › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › Inducing a Mucosal Barrier-Sparing...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs de reconnaissance de motifs moléculaires › Récepteurs de type Toll › Récepteur de type Toll-3 › Inducing a Mucosal Barrier-Sparing...