Large-cage assessment of a transgenic sex-ratio distortion strain on populations of an African malaria vector.
Anopheles gambiae
Genetic control
Genetic load
Malaria
Male-bias
Population model
Validation
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
06 Feb 2019
06 Feb 2019
Historique:
received:
25
05
2018
accepted:
03
01
2019
entrez:
8
2
2019
pubmed:
8
2
2019
medline:
20
2
2019
Statut:
epublish
Résumé
Novel transgenic mosquito control methods require progressively more realistic evaluation. The goal of this study was to determine the effect of a transgene that causes a male-bias sex ratio on Anopheles gambiae target populations in large insectary cages. Life history characteristics of Anopheles gambiae wild type and Ag(PMB)1 (aka The frequency of transgenic mosquitoes in target populations reached an average of 0.44 ± 0.02 and 0.56 ± 0.02 after 6 weeks in the 1:1 and in the 3:1 release ratio treatments (transgenic male:wild male) respectively. Transgenic males caused sex-ratio distortion of 73% and 80% males in the 1:1 and 3:1 treatments, respectively. The number of eggs laid in the transgenic treatments declined as the experiment progressed, with a steeper decline in the 3:1 than in the 1:1 releases. The results of the experiment are partially consistent with predictions of the model; effect size and variability did not conform to the model in two out of three trials, effect size was over-estimated by the model and variability was greater than anticipated, possibly because of sampling effects in restocking. The model estimating the effects of hypothetical releases on the mosquito population of a West African village demonstrated that releases could significantly reduce the number of females in the wild population. The interval of releases is not expected to have a strong effect. The biological data produced to parameterize the model, the model itself, and the results of the experiments are components of a system to evaluate and predict the performance of transgenic mosquitoes. Together these suggest that the Ag(PMB)1 strain has the potential to be useful for reversible population suppression while this novel field develops.
Sections du résumé
BACKGROUND
BACKGROUND
Novel transgenic mosquito control methods require progressively more realistic evaluation. The goal of this study was to determine the effect of a transgene that causes a male-bias sex ratio on Anopheles gambiae target populations in large insectary cages.
METHODS
METHODS
Life history characteristics of Anopheles gambiae wild type and Ag(PMB)1 (aka
RESULTS
RESULTS
The frequency of transgenic mosquitoes in target populations reached an average of 0.44 ± 0.02 and 0.56 ± 0.02 after 6 weeks in the 1:1 and in the 3:1 release ratio treatments (transgenic male:wild male) respectively. Transgenic males caused sex-ratio distortion of 73% and 80% males in the 1:1 and 3:1 treatments, respectively. The number of eggs laid in the transgenic treatments declined as the experiment progressed, with a steeper decline in the 3:1 than in the 1:1 releases. The results of the experiment are partially consistent with predictions of the model; effect size and variability did not conform to the model in two out of three trials, effect size was over-estimated by the model and variability was greater than anticipated, possibly because of sampling effects in restocking. The model estimating the effects of hypothetical releases on the mosquito population of a West African village demonstrated that releases could significantly reduce the number of females in the wild population. The interval of releases is not expected to have a strong effect.
CONCLUSIONS
CONCLUSIONS
The biological data produced to parameterize the model, the model itself, and the results of the experiments are components of a system to evaluate and predict the performance of transgenic mosquitoes. Together these suggest that the Ag(PMB)1 strain has the potential to be useful for reversible population suppression while this novel field develops.
Identifiants
pubmed: 30728060
doi: 10.1186/s13071-019-3289-y
pii: 10.1186/s13071-019-3289-y
pmc: PMC6366042
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
70Références
J Med Entomol. 2002 Jan;39(1):162-72
pubmed: 11931252
Science. 2002 Oct 4;298(5591):117-9
pubmed: 12364785
Nat Biotechnol. 2005 Nov;23(11):1414-7
pubmed: 16244659
BMC Biol. 2007 Mar 20;5:11
pubmed: 17374148
Genetics. 2008 Aug;179(4):2013-26
pubmed: 18660532
J Insect Physiol. 2009 Mar;55(3):197-204
pubmed: 19041323
PLoS Genet. 2008 Dec;4(12):e1000291
pubmed: 19057670
Malar J. 2009 Nov 16;8 Suppl 2:S2
pubmed: 19917072
J Am Mosq Control Assoc. 2011 Sep;27(3):227-35
pubmed: 22017086
Parasit Vectors. 2012 Apr 04;5:69
pubmed: 22475528
J Med Entomol. 2012 May;49(3):595-605
pubmed: 22679867
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):E1922-30
pubmed: 22689959
Parasit Vectors. 2012 Jun 19;5:122
pubmed: 22713553
J Med Entomol. 2012 Sep;49(5):1001-11
pubmed: 23025180
J Anim Ecol. 2013 Jan;82(1):166-74
pubmed: 23163565
J Med Entomol. 2012 Nov;49(6):1177-88
pubmed: 23270145
J Vis Exp. 2014 Jan 04;(83):e3579
pubmed: 24430003
Proc Natl Acad Sci U S A. 2014 May 27;111(21):7600-5
pubmed: 24821795
Philos Trans R Soc Lond B Biol Sci. 2014 May 12;369(1645):20130432
pubmed: 24821918
Nat Commun. 2014 Jun 10;5:3977
pubmed: 24915045
Math Biosci. 2014 Aug;254:6-27
pubmed: 24929226
J Appl Ecol. 2013 Oct;50(5):1216-1225
pubmed: 25558082
Malar J. 2015 Jul 15;14:271
pubmed: 26169677
Proc Natl Acad Sci U S A. 2015 Dec 8;112(49):E6736-43
pubmed: 26598698
Nat Biotechnol. 2016 Jan;34(1):78-83
pubmed: 26641531
Trends Parasitol. 2016 Mar;32(3):219-229
pubmed: 26897660
Malar J. 2016 May 10;15(1):262
pubmed: 27160438
Parasit Vectors. 2016 May 10;9(1):272
pubmed: 27165292
Parasit Vectors. 2016 Aug 12;9(1):446
pubmed: 27519419
Insects. 2016 Sep 22;7(4):null
pubmed: 27669312
Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):E255-E264
pubmed: 28028208
Parasit Vectors. 2017 Aug 7;10(1):376
pubmed: 28784147
BMC Biol. 2017 Sep 11;15(1):81
pubmed: 28893259
PLoS Pathog. 2018 Mar 8;14(3):e1006898
pubmed: 29518156
Malar J. 2018 Apr 2;17(1):140
pubmed: 29609598
Med Vet Entomol. 2019 Mar;33(1):1-15
pubmed: 30044507
Nat Biotechnol. 2018 Dec;36(11):1062-1066
pubmed: 30247490
J Hered. 1979 Sep-Oct;70(5):345-6
pubmed: 43343
Bull World Health Organ. 1968;38(3):421-38
pubmed: 5302334
Annu Rev Entomol. 1984;29:137-56
pubmed: 6362549