PLK1 plays dual roles in centralspindlin regulation during cytokinesis.
Animals
Aurora Kinase B
/ genetics
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ genetics
Cell Cycle Proteins
/ genetics
Cytokinesis
Enzyme Activation
HeLa Cells
Humans
Microtubule-Associated Proteins
/ genetics
Microtubules
/ enzymology
Phosphoproteins
/ genetics
Protein Serine-Threonine Kinases
/ genetics
Protein Transport
Proto-Oncogene Proteins
/ genetics
Signal Transduction
Spindle Apparatus
/ enzymology
rhoA GTP-Binding Protein
/ genetics
Polo-Like Kinase 1
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
08
05
2018
revised:
26
12
2018
accepted:
23
01
2019
pubmed:
8
2
2019
medline:
14
4
2020
entrez:
8
2
2019
Statut:
ppublish
Résumé
Cytokinesis begins upon anaphase onset. An early step involves local activation of the small GTPase RhoA, which triggers assembly of an actomyosin-based contractile ring at the equatorial cortex. Here, we delineated the contributions of PLK1 and Aurora B to RhoA activation and cytokinesis initiation in human cells. Knock-down of PRC1, which disrupts the spindle midzone, revealed the existence of two pathways that can initiate cleavage furrow ingression. One pathway depends on a well-organized spindle midzone and PLK1, while the other depends on Aurora B activity and centralspindlin at the equatorial cortex and can operate independently of PLK1. We further show that PLK1 inhibition sequesters centralspindlin onto the spindle midzone, making it unavailable for Aurora B at the equatorial cortex. We propose that PLK1 activity promotes the release of centralspindlin from the spindle midzone through inhibition of PRC1, allowing centralspindlin to function as a regulator of spindle midzone formation and as an activator of RhoA at the equatorial cortex.
Identifiants
pubmed: 30728176
pii: jcb.201805036
doi: 10.1083/jcb.201805036
pmc: PMC6446842
doi:
Substances chimiques
CYK-4 protein, C elegans
0
Caenorhabditis elegans Proteins
0
Cell Cycle Proteins
0
Microtubule-Associated Proteins
0
PRC1 protein, human
0
Phosphoproteins
0
Proto-Oncogene Proteins
0
SPD-1 protein, C elegans
0
spindlin
0
RHOA protein, human
124671-05-2
AURKB protein, human
EC 2.7.11.1
Aurora Kinase B
EC 2.7.11.1
Protein Serine-Threonine Kinases
EC 2.7.11.1
rhoA GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
1250-1264Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM085087
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127091
Pays : United States
Informations de copyright
© 2019 Adriaans et al.
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