The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.
Amino Acid Sequence
Animals
Aspartic Acid Endopeptidases
/ chemistry
Calcium
/ metabolism
Calcium-Binding Proteins
/ metabolism
Cell Membrane
/ enzymology
Endoplasmic Reticulum
/ metabolism
Female
Germ Cells
/ metabolism
HEK293 Cells
HeLa Cells
Homeostasis
Humans
Male
Membrane Proteins
/ chemistry
Mice
Organ Specificity
Spermatids
/ metabolism
Substrate Specificity
Testis
/ enzymology
intramembrane proteolysis
phospholamban
signal peptide peptidase‐like proteases
spermatogenesis
tail‐anchored proteins
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
18
05
2018
revised:
21
12
2018
accepted:
21
12
2018
pubmed:
9
2
2019
medline:
28
4
2020
entrez:
9
2
2019
Statut:
ppublish
Résumé
Signal peptide peptidase (SPP) and the four homologous SPP-like (SPPL) proteases constitute a family of intramembrane aspartyl proteases with selectivity for type II-oriented transmembrane segments. Here, we analyse the physiological function of the orphan protease SPPL2c, previously considered to represent a non-expressed pseudogene. We demonstrate proteolytic activity of SPPL2c towards selected tail-anchored proteins. Despite shared ER localisation, SPPL2c and SPP exhibit distinct, though partially overlapping substrate spectra and inhibitory profiles, and are organised in different high molecular weight complexes. Interestingly, SPPL2c is specifically expressed in murine and human testis where it is primarily localised in spermatids. In mice, SPPL2c deficiency leads to a partial loss of elongated spermatids and reduced motility of mature spermatozoa, but preserved fertility. However, matings of male and female
Identifiants
pubmed: 30733280
pii: embr.201846449
doi: 10.15252/embr.201846449
pmc: PMC6399600
pii:
doi:
Substances chimiques
Calcium-Binding Proteins
0
Membrane Proteins
0
phospholamban
0
Aspartic Acid Endopeptidases
EC 3.4.23.-
SPPL2C protein, human
EC 3.4.23.-
Sppl2c protein, mouse
EC 3.4.23.-
signal peptide peptidase
EC 3.4.23.-
Calcium
SY7Q814VUP
Banques de données
GENBANK
['PXD011922']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 The Authors.
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